Poly Cystic Ovary Model as an Elevated Oxidative Stress Factor

Background and Polycystic ovary syndrome (PCOS) is one of the most common causes of infertility in modern societies. The etiology of PCOS is unknown, so a good animal model can be valuable in studying its pathogenesis and could help in making a more effective diagnosis and choosing appropriate treatments. All PCOS disorders may be the result of multiple genetic and environmental factors. One of these environmental factors is reactive oxygen species (ROS). The aim of this study was to make oxidative stress by induction of polycystic ovary model. PCOS was induced in 30 NMRI mature female mice during 8 weeks which were divided into a control group and an experimental group. PCOS was induced by single injection of estradiol valerate (40 mg/kg). After PCOS induction, body weight was measured weekly for 8 weeks. Mice in each group were scarified and their ovarian tissues were collected. Histopathological study was done to confirm the model. Oxidative stress and antioxidant capacity levels were measured in ovarian tissue using Flow cytometric and Fluorometric techniques. Data was analyzed by independent sample T-test. Both groups showed significant increase in weight at the end of the study (P≤0.05). Histopathological studies confirmed PCO model. Flow cytometric analysis revealed that oxidative stress significantly increased in ovarian tissue in PCO group, (P≤0.05). Also, in TAC study of ovarian tissue no significant difference was observed between the two groups. According to this study, PCO can increase oxidative stress in ovarian tissue. This increase could make more cysts in mouse ovarian tissue.