Effects of Methanol Spraying on Qualitative traits, Yield and Yield Components of Soybean (Glycine max L.) under Drought Stress Conditions

IntroductionSoybean (Glycin max L.) is one of the most important oilseed crops in the world. It can provide oil and vegetable protein suitable for feeding humans as well as animals. The productivity Increasing of this crop in Iran has been the subject of continuous investigation over the past few years. It is well known that adequate water supply is considered as a very important factor to affect the accumulation of dry matter in the plant as well as vegetative growth of most crops. Irrigation is an important factor affecting soybean growth and yield and its related components. Exposing soybean plants to soil moisture stress at any phase of its life cycle may lead to a detrimental effect on growth, yield and its components. The methanol spraying can lead to increase in yield, expediting in maturity and reduction in drought stress impacts and water requirement of crops.
Material and MethodsThe experiment was conducted as split plots based on randomized complete block design with three replications at the Research Farm, Faculty of Agriculture of Moghan, Iran, in 2011. Treatments included three levels of drought stress as follows irrigation after, 40 (control), 55 and 70 percentage of available soil moisture depletion as main plots, and four levels of methanol spraying including 0 (control), 7, 21 and 35 volumetric percentage as sub plots. The studied traits were included plant height, leaf area, number of pod and seed per plant, 1000 seed weight, biological and seed yield, stomatal conductance and proline contents. Statistical analysis was carried out using SAS version 9.1 software. Significant difference was set at p ≤ 0.05 by using Duncan’s multiple range test.
Results and DiscussionThe results showed that the plant height, leaf area, number of pod and seed per plant, 1000 seed weight, biological and seed yield, stomatal conductance and proline contents as well as number of leaf per plant significantly affected by drought stress and methanol spraying. By increasing drought stress, proline content increased, while other traits were decreased and 70 percentage of available soil moisture depletion decreased the seed yield by 51.2%over than the control. With increasing of methanol spraying to 21 of volumetric percentage, all the investigate traits, except the proline content increased and increasing the volumetric percentage methanol more than it, was reduces them. The maximum amounts of quantity traits and stomatal conductance of soybean obtained at 21 volumetric percentage of methanol spraying, and the seed yield of this treatment was 25.6%higher than the control . These results are in agreement with those obtained by Purmousavi et al., (2009), Ruhul Amin et al., (2009), Shahmoradi et al., (2009) and Ibrahim and Kandil, (2007), who found that deficit irrigation, caused a significant decrease in yield and yield components of soybean. In general, irrigation decreasing may be lead to reducing photosynthesis activity and induce imbalanced relations between plant hormones and biological processes in the plant organs as a whole. These conditions in the treated soil are undoubtedly of great importance throughout the vegetative growth and dry matter accumulation in soybean plants. Drought stress reduces leaf size, stem extension and root proliferation; it disturbs plant water relations as well as dry matter production (Farooq et al., 2009).
ConclusionsIn general, the results of experiment showed that the maximum amounts of quantitative traits, stomatal conductance and proline content of soybean, obtained by 21 volumetric percentage of methanol spraying and by this treatment, the seed yield was 25.6 % higher than control.