Comparison of Head Space Solid Phase Microextraction (HS-SPME) and Hydro-distillation (HD) Techniques in Determination of Essential Oils in Fritillaria imperialis

Fritillaria imperialis is an endangered species with pharmaceutical alkaloids that can be effective in reducing blood pressure and reducing cardiac palpitations.   Comparison of head space solid phase microextraction (HS-SPME) and hydro-distillation (HD) techniques in determination of essential oils in Fritillaria imperialis Quality and quantity determination of components of extracted essential oils from aerial parts of Fritillaria imperialis by Head space-Solid phase microextraction (HS-SPME) and hydro-distillation (HD) techniques using Gas Chromatography-Mass Spctroscopy (GC-MS) method. 24 components (86.61%) were identified in the oil was isolated by hydrodistillation. The major components of oil of F.imperialis were α-Bisabolol oxide A (28.2%), Camphor (12.86%), Chamazulene (11.8%), Trans-Thujone (10.9%), α-Bisabolone oxide A (10.3%), δ-3-Carene (6.9%) and α-Pinene (5.65%). 21 components (94.93%) were characterized in the oil which was obtained by Head space-solid phase microextraction (HS-SPME). The major components of the oil were α-Bisabolol oxide A (35.2%), Camphor (9.54%), Chamazulene (18.3%), Trans-Thujone (7.89%), α-Bisabolone oxide A (11.8%), δ-3-Carene (6.5%) and α-Pinene (5.7%).   In both methods (HD & HS-SPME), oxygenated sesquiterpenes were the main components of the oil, whereas hydrocarbon sesquiterpens were trace.