Isolation of Multiple Drug-Resistant Genes on Encoding Exofoliative Toxin B Plasmid by Multiplex-PCR in Staphylococcus aureus Skin Infection Samples

Exfoliative toxin is Staphylococcus aureus various factors that can caused colonization and virulance. The aim of this study was to investigate the presence of several antibiotic resistant genes on exfoliative B-encoding plasmids in S. aureus infected skin specimens. 100 wound samples collected from Tehran Hospitals. Bacteria were identified by standard biochemical and microbiological tests. Antibiogram test was performed for S. aureus isolates. DNA was extracted of resistance S. aureus. Presence of antibiotic resistant etb, aac, and msrA genes were identified by Muliplex-PCR. From 100 samples, 60 samples were S. aureus. Resistance to erythromycin, gentamicin, tobramycin, ciprofloxacin and linzolide was 3 (5%), 1 (2%), 1 (2%), 1 (2%) and 0 (0%) respectively. Frequency of msrA, aac and etb genes were 29 (48.3%), 45 (75%) and 37 (61.7%) respectively. Frequency for simultaneously presence of the etb / aac, etb / msrA, msrA / aac and msrA / aac / etb gene were 28 (46.6%), 22 (36.6%), 22 (36.6%) and 18 (30%) strains. . In this study, etb gene was indicated in most strains. As also, investigation of virulence genes in S. aureus skin infection samples showed frequency of resistance pathogen genes were increased.