Effect of Saccharomyces Cerevisiae Supplementation on In Vitro Gas Production of Biscuit Waste

An in vitro gas production technique was used in this study to elucidate the effect of active live yeast on gas production. The treatment contained 0, 2.5, 5 and 7.5 g yeast Saccharomyces cerevisiae (Sc) per kg of biscuit waste based on DM, respectively. 2 h after incubation time the treatment with Sc 7.5 g/kg DM had the highest gas production (P<0.05). At the early incubation times (4 and 6 h), the treatments 1 and 2 (treatment with Sc, 0 and 2.5 g/kg DM, respectively) had the highest gas production volume among treatments, but up to 8 h the gas production volume in treatment 4 (treatments with Sc, 7 g/kg DM) was the lowest gas production volume (p<0.05). It was concluded that in vitro gas production valueof biscuit waste was improved with addition of yeast Saccharomyces cerevisiae at 2.5 g/kg DM, but Saccharomyces cerevisiae at 5 and 7.5 g/kg DM decreased gas production volume.