Antimicrobial activity Kefir on different time fermentation

Kefir is a probiotic mixture of bacteria and yeast originating from Qafqaz region.The Kefir  grain contain both Lactic acid bacteria (Lactobacillus, Lactococcus, Leuconostoc, Acetobacte and
Streptococcus spp.) and yeast (Kluyveromyces,Torula, Candida and Saccharomyces spp.). Kefir  is claimed to have therapeutic effect.This study looked at the antimicrobial activity of Kefir on
Pseudomonas aeruginosa.

The antimicrobial activity of Kefir extract were determined on Pseudomonas  aeruginosa  (ATCC 27853) and on those isolated from burned patient. The MIC was defined as the lowest
antimicrobial concentration able to completely inhibite bacterial growth up to 24 h. MIC values  were determined by microdilution method. The lactic acid and acetic acid contents of the Kefir
extracts were determined by reverce-phase HPLC.

The result showed that the highset antimicrobial activity of Kefir exteract on Pseudomonas aeruginosa (ATCC 27853) and the burned patient isolate ranged from 250  mg/mL(MIC) to 250 mg/mL(MBC) on time 96h.

The Kefir exteract showed significant antimicrobial activity on Pseudomonas aeruginosa (ATCC 27853) and the clinical isolate of Pseudomonas aeruginosa.