DNA Extraction from dried specimens of plant and fungi in a fast, secure and non-use liquid nitrogen method

The use of various molecular techniques to improve plants and fungi requires high quality DNA extraction. In most plant and fungal DNA isolation methods, common techniques that are used include the use of CTAB, enzymes (such as proteinase K), toxic solvents (such as phenol), liquid nitrogen, SDS, or the use of DNA extraction kits each have their own advantages and disadvantages. In most DNA extraction procedures, it is necessary to use liquid nitrogen to make powdered plant or fungi material. It is recommended that no liquid nitrogen be used in closed spaces because its evaporation causes an increase in nitrogen levels in the air, resulting in a decrease in oxygen that can cause headaches, anesthesia or even death. Contact with liquid nitrogen causes severe burns, which can lead to death of organs or even death. On the other hand, the sample of the fungus or plant should be transferred to the laboratory in an ice box, which requires a sample transfer equipment and refrigerator at -40 ° C. In this research, after collecting leave of wheat and Rhizoctonia solani samples, we first put them in unopened paper bags at a temperature of 25 to 30 ° C in the oven and dried. Two type extraction buffers were prepared and extraction of heredity materials from the samples was done according to the instructions provided and its quantity and quality was evaluated using spectrophotometric apparatus and using ISSR primers by polymerase chain reaction, respectively. The method presented in this study is a fast, secure, economic and simple approach. This instruction is very useful for isolating DNA from leaves of species of cereals and fungal species similar to rhizoctonia. The results showed that the quality and quantity of extracted DNA was high and its multiplication was very well.