The effect of brine and drying temperature on the functional properties of protein isolates extracted from sesame meal

As the demand for new protein sources increases, research is needed to extract and develop protein isolates with desirable functional properties. Sesame protein can be used as a new plant protein source due to its high amino acid content. In this study, the effect of sodium chloride salt concentration (0, 18 and 22% w / v) and drying temperature (25, 45 and 180 °C) as two important parameters affecting the functional properties of protein isolates extracted from sesame were investigated. Protein solubility tests at pH 4, 5, 7, 9 and 11, foam stability and emulsion stability at pH 11 and water holding capacity (WHC) and oil holding capacity (OHC) were performed. The lowest protein solubility was observed at pH 5 and with increasing pH, protein solubility increased. With increasing temperature from 45 to 180 °C at constant salt concentration, protein solubility and foam and emulsion stability increased and WHC and OHC decreased. An increasing in salt concentration from 18 to 22 % at constant temperature caused to increase in protein solubility and decrease in WHC and OHC. Foam and emulsion stability was lowest at 18% of salt concentration. In general, drying temperature of 45 degrees Celsius and salt concentration of 18% for preparation of protein isolate with maximum water and oil holding capacity and drying temperature of 180 °C and salt concentration of 22% for production of sesame protein isolate with maximum solubility, Emulsion stability and foam stability are recommended.