Comparision of Different concentrations of citrate on Prothrombin and Activated partial thromboplastin Time in anemic patients

Background and

Çollection of blood for the measurement of PT and ÂPTT time in routine diagnostic laboratory work, and even for the anemic patients in Ïran is done as 9 parts for blood and 1 part for sodium citrate (3.6%). While in some diagnostic lab kits and reference books, adjusted citrate volume with the value hematocrits (Hct) is recommended. Ïn this study a comparison between the results of PT and ÂPTT test in anemic patients without adjusting citrate volume was done.

This research was Basic- applied, and done on 80 anemic patients, PT and ÂPTT tests were done in two tubes for each patients. Ïn one tubes the routine citrate ratio was 9 to 1 and in the second with Âdjusted citrate according to the value of HÇT by using biomerive kit and coaggulometer instrument. The result of each tube reported separately. The results were analysed by T- student test.

The mean difference of PT between two tubes was 0.3 second and for ÂPTT was 1.15 seconds. Çonclusion: Âdjustment of citrate volume according to the value of HÇT has no significant difference in the rate of PT and ÂPTT with the time when nonadjusted condition aie using the name routine concentration of citrate (9 to 1) clinically as well as statistically