Studyo f GeneticD iversifyo f Raeinic ashmereG oatB asedo n Microsatellite Markers

A studyw as caniedo ut to determinet he geneticd iversityo f Raeini Cashmereg oat,u sing eight micro satellitem arkersA. numbero f wholeb lood samplesw erer andomlyc ollectedf rom 120i ndividual goats (male and female). Genomic DNA was isolated using a salting-out method with some modificationsA. ll of the loci concemeda ndt heys howedp olymorphismb etween6 - 12 allelesF. ive studiedlo ci (l.SCVll. OraFCB20,I LSTS059,I L2RA, ILSTS034)w ere at Hardy-Weinberg disequilibrium(P <0.05)H. eterozygosivtya riedf rom 0.7607fo r IL2RA to 0.8766f or OraFCB20lo cus. Within eightp olymorphiclo ci, theset wo loci hadt he lowesta ndt he highestd iversity,r espectively. The studyo f Shannonin dex andP IC (PolymorphicIn formationC ontent)a lsoi ndicatedth e lowest andt he highestd iversityf or IL2RA andO raFCB2Olo ci, respectivelyA. verageh eterozygositwy as estimateda s 0.80 for this population.I t could be concludedth at Raeini Cashmereg oat has high geneticd iversity.