Medical Laboratory Journal
Volume:7 Issue: 1, 2013

Enterococci are Gram-positive members of human gastrointestinal flora،in Dairy products and environment. they have emerged as important causes of opportunistic nosocomial infections in recent years. In this study we aimed to investigat and compare the efficiency of MALDI-TOFmass spectroscopy method through Biochemical and Molecular methods for detecting Enterococcus faecalis and Enterococcus faecium.

seventhy five clinical samples were collected for biochemical، molecular and mass spectroscopy investigations. Samples were treated with Esculin hydrolysis، Catalase، Pyrrolidonylaminopeptidase، 6. 5% NaCl solution، motility، 0. 04% Tellurite، L-Arabinose and Sorbitol. Using specific primesallele specific PCR was used. The samples were then analyzed by MALDI-TOF mass spectroscopy and Biotyper 3 software.

Enterococcus faecium andEnterococcus faecaliswere detected in thirty and forty two samples، respectively whereas three samples showed both bacterial infections. Using biochemical analysis، two E. faecium isolates were Arabinose negative and one E. faecalis isolates was Telliurite negative. All sampleswere showed correct bands in PCR results but twoof them didn''t show clear bands (on agarose gel). In mass spectroscopy analysis all strains were correctly detected and well defined.

According to our results، MALDI-TOF mass spectrometry in comparison with Molecular and Biochemical Methodscould be a reliable and accurate method that can easily and quickly identify and differentiate Enterococcus faecium and Enterococcus faecalisin clinical samples.

Irritable bowel syndrome (IBS) is one of the most common gastrointestinal diseases. It is a functional bowel disorder characterized by chronic abdominal pain and alternation of bowel habits with no structural abnormality. The prevalence of this disease was estimated 10-20 percent in the world. However، the cause of IBS is still unknown. Regarding to intestinal absorption disorder of nutrients، the malabsorption of vitamin may occur. The aim of this study is to evaluate serum levels of vitamins A and E in IBS patients.

This case - control study was done on 94 patients whom their IBS disease were confirmed by a gastroenterologist in Golestan province. The control group was selected from healthy people، who didn''t show any signs of digestive problems in past two years. The age and sex were matched with the cases group. Using HPLC method، Fasting blood samples were collected. Followed by measurement of Serum levels of vitamins A and E.

the meanserum levels of vitamin A and E in patients and control group were 57. 0 ± 114. 8μg / dl and23. 8 ± 55. 9 μg / dl، and 0. 50 ± 0. 24 mg / dl and 1. 93 ± 1. 86 mg / dl، respectively. (P <0. 05). in men، the deficiency of Vitamin A and E، were7. 70 and 7. 10 percent and 6. 76 and 3. 7 in women. (P <0. 05). In general، 1. 1percent of IBS patients showed Vitamin A deficiency and 93. 6 percent of them had vitamin E deficiency (P <0. 05).

In this study، a significant decrease in vitamin E levels was observed in patients with IBS. Due to antioxidant activity of vitamin E، the deficiency of this Vitamin، can increase the oxidative factors leading to intestinal damages and it is expected to decrease the amount of vitamins، subsequently

Every organization requires ongoing evaluation of existing conditions. The purpose of this study is to assess and analyze the standards and criteria that each Laboratory system is required to observe and upgrade them، to determine the gap between the ideal and the current status and finally to present the strategy and executive plan in order to achieve the desirable status.

This study was performed in a medical diagnostic laboratory in Gorgan by using the quality system checklist related to medical diagnostic laboratories، which was revised in 2009. Internal evaluation matrix (Internal Factor Evaluation) was used to examine the main factors in the context of establishing a quality management system in a clinical laboratory. After examining the factors، determining the laboratory status، recording the results of monitoring (in terms of strengths and weaknesses) and determining the gap between existing and desirable status، we provided the appropriate and effective solutions in line with defined standard.

of 164 items thatshould have been done in the first assessment، 111 (67. 7%) items are in performed group and 53 (32. 3%) are not in. After compiling and running a plan، 147 (89. 6%) are performed، 15 (9. 2%) needed to be modified and 2 (1. 2%) still not performed. It is evident that a significant difference (p< 0. 05) and a tangible improvement in current problems are seen after establishing the qualitysystem in the laboratory. The lab equipment، lab space and facilities، pre-examination process، testing process and post-examination process are considered. Regarding the health and safety in the laboratory، lab equipment، lab space and facilities، pre-examination process، testing process and post-examination process، no significant difference is observed between before and after the implementation of the program.

after establishing the quality system in laboratory، a significant difference and tangible improvement in the current problems are observed. It is implied the importance of pre-planned responses to problems and the performance of strategic planning.