دکتر محمود ابراهیمی

Obesity has been managed using different treatments including cryolipolysis; while a relationship was reported between obesity and mental disorders too. This study aimed to assess the effect of low caloric diet plus cryolipolysis on depression and anxiety in comparison to low caloric diet alone in overweight subjects. In a randomized controlled clinical trial, 50 healthy overweight females (25 kg/m2≤ body mass index (BMI)<30 kg/m2) aged 18 to 65 years were recruited in this study. Subjects were randomly allocated to two groups who received a calorie restricted diet with and without cryolipolysis. Anthropometrics and serum biochemistry tests were undertaken to measure the baseline and the end of the study (8th week). Depression and anxiety were assessed using the body shape questionnaire (BSQ), Beck’s depression inventory (BDI) and Beck Anxiety Index (BAI) tests at the beginning and the end of study. All participants completed the study period. A significant difference was found in the BSQ at the baseline and the end of study between the intervention and control groups (9.96±18.61 vs. -19.24±26.55, respectively, p<0.001). A significant difference was found in BAI (-3.52±7.63 vs. 1.08±7.01, respectively, p=0.031) between the two groups. BDI changes between the two groups did not differ significantly (p>0.05). Cryolipolysis was demonstrated to improve the anxiety in overweight subjects. The molecular mechanism is not clear yet and further studies with a large sample size are necessary to be investigated.

The development of type 2 diabetes mellitus (T2DM) is associated with lifestyle factors, including dietary patterns. A diet rich in macro- and micronutrients has been reported to reduce the risk of T2DM. Therefore, this study aimed to identify the dietary factors most closely associated with T2DM in subjects within the MASHAD cohort using a decision tree algorithm.  This cross-sectional study was conducted on 9704 individuals from the Mashhad Stroke and Heart Atherosclerotic Disorders (MASHAD), of whom 5936 participants completed a 24h dietary recall questionnaire. Macronutrients and micronutrients were estimated using Diet Plan 6 software. A decision tree algorithm was utilized to evaluate the most crucial dietary nutrient intakes concerning T2DM.  The algorithm showed a high specificity (81.34%) but low sensitivity (34.21%), which could predict T2DM with a low-to-moderate diagnostic ability (AUC=0.58). Based on the decision tree, eight features, including dietary potassium, total sugar, sucrose, riboflavin, thiamin, sodium, total nitrogen, and magnesium, were T2DM’s most critical dietary components.  Based on the results, consuming sugar, salt, and vitamin B was the most critical related dietary intake to T2DM. Dietary interventions may be a cost-effective strategy for preventing T2DM.

It has been shown that angioplasty and endovascular stent deployment, used after coronary revascularization, are associated with an inflammatory response. Inflammation has a key role in the complications of atherosclerotic plaque, coronary artery disease (CAD) and in-stent restenosis (ISR). The objectives of the present study was to investigate serum levels of 12 pro/anti-cytokines and growth factors and their relationship with restenosis.

A total of 244 subjects were recruited in current study including unrelated patients who previously underwent coronary stent implantation (between 2014 and 2017) and were subsequently indicated for coronary angiography. According to angiography results patients were allocated into two groups: cases with stenosis more than 50% within the stent (N=79) and controls with stenosis less than 50% within the stent (N=165). Serum was separated by centrifuging the blood for 15 min at 1000 rpm.  Serum cytokines levels including IL-1α, IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, TNF-α, IFN-γ, MCP-1, EGF, and VEGF were measured using an EV 3513 cytokine biochip array (Randox Laboratories, Crumlin, UK).

The mean age of the NISR and ISR groups were 62.47±9.2 and 59.49±8.48 years, respectively. The diabetes frequency was significantly higher in the ISR group (55.1%) compared with NISR group (30.9%) (p<0.001). There was no significant difference in levels of cytokines between the two groups (p>0.05).

The results showed that serum levels of pro/anti-inflammatory cytokines and growth factors did not have a significant difference between NISR and ISR study groups.

Diabetes (DM) is a type of metabolic disorder that its types are generated by collectingof genetic and environmental risk agents. Here, the association between HSPB1 polymorphism as a genetic risk factor and DM was investigated.
Total 690 participants from MASHAD cohort study population were recruited into the study.Anti-HSP27-level was assessed followed by genotyping using Taqman®-probes-based assay. Anthropometric, demographic and hematological/biochemical characteristics were evaluated. Kaplan-Meier curves were utilized, while logistic regression models were used to assess the association of the genetic variant with clinical characteristics of population.
Finds was shown there are meaningful differences among groups of age, height, waist circumference, systolic blood pressure, FBG,TG, HDL-C, and hs-CRP, and was no big -significant difference between theexists in different HSP27 SNP in the two studied groups (with and without DM), also was no remarkable relation between genetic forms of HSPB1and T2DM. This investigation was the first research that analyzed the relationship between the genetic type of the HSPB1 gene (rs2868371) and Type 2 diabetes (DM2). In our population, the CC genotype (68.1%) had a higher prevalence versus GC (26.6%) and GG (5.3%) genotypes and the data shown that no genetic difference of HSPB1 gene polymorphism (rs2868371) was related with DM2.
HSPB1 polymorphism, rs2868371, was not associated with type 2 diabetes mellitus.

familial hypercholesterolemia (FH), a hereditary disorder, is caused by pathogenic variants in the LDLR, APOB, and PCSK9 genes. This study has assessed genetic variants in a family, clinically diagnosed with FH.

A family was recruited from MASHAD study in Iran with possible FH based on the Simon Broom criteria. The DNA sample of an affected individual (proband) was analyzed using whole exome sequencing, followed by bioinformatics and segregation analyses.

A novel splice site variant (c.345-2A>G) was detected in the LDLRAP1 gene, which was segregated in all affected family members. Moreover, HMGCR rs3846662 g.23092A>G was found to be homozygous (G/G) in the proband, probably leading to reduced response to simvastatin and pravastatin.

LDLRAP1 c.345-2A>G could alter the phosphotyrosine-binding domain, which acts as an important part of biological pathways related to lipid metabolism.