ahmed fayed

Medical renal diseases stand as one of the major health problems in pediatric age group considering its morbidity/mortality and the subsequent management plans.

In this manuscript, the spectrum of histopathological patterns of medical nephropathic lesions in Egyptian pediatric patients over duration of five years is reported with clinical indications. Patients and

We conducted a retrospective study for analysis of our pathological reports of renal needle biopsies during the period from January 2014 until January 2019. One hundred and sixteen cases were included.

The most commonly encountered pediatric renal pathology was minimal change disease (27.59%), followed by congenital glomerular diseases (22.41%), focal segmental glomerulosclerosis (12.93%), and thrombotic microangiopathy (7.76%). The most common clinical indication for biopsy was nephrotic syndrome (37.07%) followed by impaired renal functions with elevated serum creatinine (21.55%). In addition, we report very rare histological findings in few cases including infantile nephropathic cystinosis, Barakat syndrome and C3 glomerulopathy.

Minimal change disease and congenital glomerular diseases accounted for half of pediatric renal pathologies in the study population. The most common clinical indication for renal biopsy was nephrotic syndrome. Electron microscopic examination and genetic studies are mandatory for proper evaluation of pediatric nephropathies.

A new differential pulse voltammetric method was developed for determination of the non-steroidal anti-inflammatory drug; acemetacin. Various experimental parameters were studied, namely; electrode type, pH of the used buffer and scan rate on the reduction and oxidation peaks of acemetacin. The drug responded only to glassy carbon electrode among the studied working electrodes with higher peak current and sensitivity in the favor of the reduction side. The results also revealed that acemetacin best assayed through measuring its reduction peak current when prepared in Britton Robinson buffer solution at pH 8 when scanned at rate of 16 mV/s. The proposed method presented a bimodal calibration curve where each segment showed strong linearity. The linearity ranges were 1 - 100 µM for the lower segment and 0.1 - 3 mM for the higher one with detection limit of 0.1 µM. The proposed method was successfully applied for determination of acemetacin in its pharmaceutical dosage form.