mohammad abdolvand

Cancer development is aided by the role of long noncoding RNAs (lncRNAs) that act as competing endogenous RNAs (ceRNAs) absorbing microRNAs (miRNAs). We aimed to discover a novel regulatory axis in colorectal cancer (CRC) and potential biomarkers based on miR‑616‑3p.

The gene expression omnibus database was mined for differentially expressed lncRNAs (DELs) and mRNAs. LncRNAs and mRNAs were predicted using the RegRNA and TargetScan databases. A combination of the ciBioPortal and Ensemble databases was used to locate the mRNAs. Cytoscape 3.7.1‑built CeRNA networks. A quantitative real‑time polymerase chain reaction (qRT‑PCR) was utilized to confirm the expression levels of these RNA molecules. Statistical analyses were implemented by GraphPad Prism 9.

qRT‑PCR showed (Linc01282, lnc‑MYADM‑1:1, and Zinc Finger Protein 347 [ZNF347]) were overexpressed whereas, (salt‑inducible kinases 1 [SIK1], and miR‑616‑3p) were down regulated.

These results identify unique, unreported lncRNAs as CRC prognostic biomarkers, as well as prospective mRNAs as new treatment targets and predictive biomarkers for CRC. In addition, our study uncovered unexplored ceRNA networks that should be studied further in CRC.

Long noncoding RNAs (lncRNAs) have been recognized as the main modulatory molecules in various cancers and perform as competing endogenous RNAs (ceRNAs). The nuclear hormone receptor superfamily of ligand?activated transcription factors (NR3C1) regulates numerous proliferative and metabolic processes such as tumorigenesis and metabolic diseases. Furthermore, X?linked inhibitor of apoptosis protein (XIAP) belongs to a family of the inhibitors of apoptosis proteins, is located downstream of the glucocorticoid receptor (GR or NR3C1) pathway, and cooperates with GR to suppress apoptosis. However, the underlying mechanisms of NR3C1 and XIAP in colorectal cancer (CRC) remain mainly unclear. This research aims to clarify the potential RNAbiomarkers and to construct a novel ceRNA network in CRC.

Multistep bioinformatics methods such as Lnc2cancer and miRDB databases were applied to identify candidate lncRNAs and miRNAs. The interaction energy between lncRNAs, NR3C1, and XIAP genes was analyzed by the LncRRIsearch database. Plus, microRNAs and lncRNA were evaluated via the Diana tools database to select microRNAs with the most binding scores. Quantitative reverse transcription– olymerase chain reaction (QRT?PCR) was applied to verify RNA molecules’ expression levels and their association with the clinicopathological factors in 30 CRC tissues compared to 30 adjacent tissues.

QRT?PCR showed upregulation of KCNQ1OT1, NR3C1, and XIAP and downregulation of miR?421. The ceRNA network was constructed with 17 lncRNAs, 2 mRNAs, and 42 miRNAs. Thus, we explained the potential interactions between KCNQ1OT1 and miR?421 with NR3C1 and XIAP genes.

Our study represents potential prognostic biomarkers and a new ceRNA network for further study in CRC.

The purpose of this study was to investigate injuries resulting from accidents and incidents referred to medical centers and hospitals of Shahid Beheshti University of Medical Sciences.
This study is a cross-sectional study. The data of all injured patients admitted to hospitals of Shahid Beheshti University of Medical Sciences from March 2014 to March 2015 were reviewed. Accidents and injuries were categorized according to the tenth version of international classification of diseases. Data collected by means of forms and software injury register by Hospital emergency department staff to interview and hospital records, were completed.
In total, 131967 cases were reviewed,in which 66% were male and 34 % were female. Traumas, traffic accidents, and falls were major causes of accidents and injuries. Accidents and injuries were more prevalent in 20-29 years old (the younger age groups; 51%). Individuals living in urban areas sustained more injuries compared to individuals from rural areas. A total of 194 deaths were reported due to accidents and injuries.
Our findings indicated that traffic accidents constitute a large proportion of accident and injuries. Productive groups (young male) were the most victims of accidents and injuries. Training of different groups, including before and during recruitment, training mothers, and elderly seems to be beneficial. Further studies to improve the registration tools for a better access to reliable data are needed. Promotion knowledge and training, especially in the younger age group, intervention to resolve the accident prone spots as well as technical defects are recommended to reduce the traffic accidents.