جستجوی مقالات مرتبط با کلیدواژه « strychnos innocua » در نشریات گروه « شیمی »

Phytosterols derived from medicinal plants are well-known for their therapeutic effects in the treatment of diabetes, cardiovascular disease, cancer, and microbial infections. Strychnos innocua (a Loganiaceae family member) grows in numerous African countries and is widely used for medicinal purposes. This plant's (root bark) ethyl acetate extract was subjected to chromatographic separation, resulting in the isolation of Campesterol (1) and β-Sitosterol (2). Their structures were verified using mass spectrometry, nuclear magnetic resonance (1D and 2D NMR), and in comparison to published data. This is a novel report of phytosterol compounds which were isolated from S. innocua root bark. The in silico investigation found that the binding affinities of Campesterol (1) with binding sites of Staphylococcus aureus pyruvate carboxylase (PDB: 3HO8) and Pseudomonas aeruginosa virulence factor regulator (PDB: 2OZ6) were -7.8 and -7.9 kcal/mol, respectively. Furthermore, the binding affinities of β-Sitosterol (2) with binding sites of S. aureus and P. aeruginosa are -7.6 and -7.7 kcal/mol, respectively, while ciprofloxacin (standard drugs) exhibited binding affinities of -6.6 and -8.7 kcal/mol. This study concluded that the S. innocua root bark has a rich presence of Campesterol and β-Sitosterol, while their molecular docking studies revealed that they have excellent interactions with S. aureus and P. aeruginosa.

Terpenoids are definite secondary metabolites with active therapeutic components that contribute to plants’ medicinal potential. Strychnos innocua is a Loganiaceae family medicinal plant found in various African countries. This study reports the isolation, characterization, and molecular docking analyses of Linalool (1) and Nerolidol (2) from ethyl acetate root bark extract of S. innocua. Their structures were validated using mass spectrometry, nuclear magnetic resonance (1D and 2D NMR), and compared with literature data. This is a novel report of terpenoids isolated from S. innocua root bark. An in silico docking examination revealed the binding energies of Linalool with the binding sites of Staphylococcus aureus pyruvate carboxylase (PDB: 3HO8) and Pseudomonas aeruginosa virulence factor regulator (PDB: 2OZ6) were -4.7 and –5.6 kcal/mol, respectively. Furthermore, the binding energies of Nerolidol with the binding sites of S. aureus and P. aeruginosa were -5.8 and -6.9 kcal/mol, respectively. Compared to ciprofloxacin (standard drug), which showed binding energies of -6.6 and -8.7 kcal/mol, respectively. This study concluded that Linalool and Nerolidol are abundant in the root bark of S. innocua. At the same time, docking results revealed that the compounds had moderate interactions with S. aureus and P. aeruginosa, exhibiting antibacterial effects.

The use of plant products as medicines could be traced as far back as the beginning of human civilization. The medicinal plant “Strychnos innocua (a Loganiaceae plant)” is a deciduous shrub growing 2-12 m tall. This study aimed at using GC-MS to determine the chemical composition and antifungal activity of a root bark methanol extract against Candida krusei. The plant sample was collected and identified at Ahmadu Bello University, Zaria. The maceration extraction method was used to obtain the methanol root bark extract. The chromatogram from the GC-MS analysis revealed sixty-four (64) peaks corresponding to fifty-five phytoconstituents from various organic groups. Among the significant compounds discovered are 9,12-Octadecadienoic acid (5.48%), Benzofuran, 6-ethenyl-4,5,6,7-tetrahydro-3,6-dimethyl-5-isopropenyl-, trans- (6.72%), Tetradecanoic acid, 12-methyl-, methyl ester (6.87%), Ethyl Oleate (8.29%), 1,2-Benzenedicarboxylic acid, butyl 8-methylnonyl ester (9.10%), Linoleic acid ethyl ester (10.31%) with 6-Octadecenoic acid, methyl ester and peak area of 11.42% being considered the highest peak area. In contrast, Bicyclo[13.1.0]hexadecan-2-one with a peak of 0.03% is considered the lowest peak area. It can be concluded that the phytocompounds discovered in the root bark methanol extract of Strychnos innocua could be valuable in treating some human ailments.

The antifungal and antibacterial effects of medicinal plants have long been known. The root bark decoction of Strychnos innocua (a Loganiaceae plant) has been used to treat skin infections, candidiasis, and other disorders. Hence, the aim of this study is to determine the phytochemical, antibacterial, and antifungal properties of root bark extracts of S. innocua. This is the first time a study of this nature is been conducted using the root bark of the plant. Maceration with n-hexane, ethyl acetate, and methanol as solvents was used in the extraction process, while the phytochemical analysis of the extracts followed a standard procedure. This confirmed the presence of flavonoids, coumarines, triterpenes, steroids, saponins, tannins, phenols, anthraquinones, quinones, carbohydrates, phlobatannins, glycosides, cardiac and alkaloids. The extracts exhibited antimicrobial activities against MRSA, B. subtilis, P. aeruginosa, S. aureus, C. krusei, A. fumigatus, C. albicans while no activity against E. coli, S. pyogenes, S. typhii, A. niger and K. Pneumoniae were observed. MIC and MBC/MFC were also determined. In conclusion, the root bark of S. innocua is thought to be rich in phytochemicals and has antifungal and antibacterial effects against some of the tested pathogens.