جستجوی مقالات مرتبط با کلیدواژه "c-reactive protein" در نشریات گروه "پزشکی"
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Background
The relationship between inflammation and pancreatic cancer (PC) has been previously explored, but the precise role of inflammatory markers in disease risk and progression remains unclear. This case-control study aimed to investigate the association between C-reactive protein (CRP), systemic inflammation marker, and dehydroepiandrosterone (DHEA), systemic cytokines regulator, in relation to pancreatic cancer risk.
MethodsSerum levels of DHEA and CRP were measured in 50 pancreatic cancer patients and 50 age and sex-matched healthy controls using enzyme-linked immunosorbent assay (ELISA) and latex particle-enhanced immunoturbidimetric assay, respectively. Data analysis was performed using STATA software.
ResultsThe results showed that while DHEA levels were lower in pancreatic cancer patients compared to healthy subjects, the difference did not reach statistical significance (p=0.74). Conversely, CRP levels were significantly elevated in pancreatic cancer patients (p=0.001). Subgroup analysis based on sex revealed significant differences in DHEA and CRP concentrations between male patients and controls. Furthermore, a marginally significant inverse relationship was observed between log CRP and DHEA levels in pancreatic cancer patients (p=0.054). Risk assessment analysis, adjusted for age and sex, demonstrated an increased risk of pancreatic cancer associated with elevated log CRP levels (p=0.001; OR=1.671), and a decreased risk associated with higher DHEA levels (p=0.024, OR=0.479).
Conclusionsour findings highlight the direct association of pancreatic cancer with CRP and the inverse relationship with DHEA, suggesting the involvement of inflammation in pancreatic cancer development. Moreover, the observed inverse correlation between CRP and DHEA among pancreatic cancer patients suggests a potential inhibitory effect of DHEA on CRP levels.
Keywords: C-Reactive Protein, Dehydroepiandrosterone, Inflammation, Risk Factors. Pancreatic Cancers -
مجله علمی دانشگاه علوم پزشکی کردستان، سال بیست و نهم شماره 4 (پیاپی 133، مهر و آبان 1403)، صص 82 -94زمینه و هدف
هدف مطالعه حاضر مقایسه فاکتورهای خونی و MRI در شرکت کنندگان +MS (با سابقه MS) و -MS (بدون سابقه MS) در راستای میزان شیوع بیماری MS در افراد -MS و افزایش شدت بیماری در +MS بود.
مواد و روش هادر مطالعه حاضر بصورت مقطعی- مشاهده ای 80 نمونه انسانی بر اساس آگهی روزنامه از افراد 20-50 ساله مبتلا به کووید-19 بر اساس تست PCR در زمان ابتلا از یکم اسفند ماه 1398 تا سی ام آبان ماه 1400 انتخاب شدند. روش تحقیق برای شرکت کنندگان توضیح داده شد و یک فرم رضایت آگاهانه را پر کرده و آن را امضا کردند. در نمونه خونی شرکت کنندگان CBC، ESR، ANA و CRP اندازه گیری شد. سپس در تمام شرکت کنندگان اسکن MRI جهت تشخیص MS انجام شد.
یافته هادر گروه های سنی 31 تا40 و 41-50، ANA به طور قابل ملاحظه ای در MS+ بیشتر از شرکت کنندگان -MS بود. ANA در MS+ بستری و سرپایی به طور قابل توجهی (05/0<p) بیشتر از -MS بستری و سرپایی بود. در مردان MS+، CRP بیشتر از مردان -MS بود. نتایج اسکن MRI نشان داد که در 13 بیماری که قبل از ابتلا به کووید-19 سابقه بیماری MS نداشته اند پلاک های MS مشاهده گردید.
نتیجه گیرینتایج نشان داد که CRP و ANA در تمام شرکت کنندگان 5/2 سال پس از عفونت کووید-19 بالاتر از حد معمول بود، اما این افزایش در شرکت کنندگان بستری و +MS بیشتر بود. همچنین MRI پلاک های MS را بعد از ابتلا به کووید-19 نشان داد.
کلید واژگان: کووید-19, مولتیپل اسکلروزیس, پروتئین واکنشی C, آنتی بادی های ضد هسته ای, سرعت رسوب گلبول های قرمزBackground and AimThis study aimed to compare blood factors and MRI in MS+ participants (with a history of MS) and MS-(without a history of MS) to determine the prevalence of MS in MS- and increase the severity of the disease in MS+.
Materials and Methods:
In this cross-sectional observational study, 80 human samples were selected from 20-50-year-olds infected with COVID-19 based on PCR tests at the time of infection from February 11th, 2020 to November 20th, 2021. The research method was explained to the participants and all of them filled out and signed the informed consent form CBC, ESR, ANA, and CRP were measured in the participants' blood samples. Then, an MRI scan was done on all participants to diagnose MS.
ResultsIn age groups 31-40 and 41-50, ANA was significantly higher in MS+ than MS- participants. ANA was meaningfully higher in inpatient and outpatient MS+ than in inpatient and outpatient MS-. In MS+ men, CRP was higher than in MS- men. The MRI scan showed that MS plaques were observed in 13 patients with no history of MS before contracting COVID-19.
ConclusionThe results showed that CRP and ANA were higher than healthy in all participants 2.5 years after the infection of COVID-19, but this increase was greater in hospitalized and MS+ participants. Also, the MRI showed MS plaques after contracting Covid-19.
Keywords: Covid-19, Multiple Sclerosis, C-Reactive Protein, Antinuclear Antibodies, Erythrocyte Sedimentation Rate -
BackgroundDexamethasone is the most widely used corticosteroid for treating COVID-19 pulmonary complications. The effects of dexamethasone on serum inflammatory factors, including C-reactive protein (CRP), lactate dehydrogenase (LDH), and cortisol, were evaluated in COVID-19 pneumonia patients with pre-existing chronic obstructive pulmonary disease (COPD) in this study.MethodsA total of 36 COVID-19 patients with pneumonia who had pre-existing COPD and were critically ill and 56 COVID-19- positive patients without pre-existing COPD were selected. A daily dose of 6–8 mg dexamethasone was administered during the hospitalization period. Some inflammatory markers (TNF-α, IL-10, and IL-6) and the serum levels of cortisol, LDH, and CRP were measured on admission and one week after hospitalization.ResultsTNF-α and IL-6 concentrations were significantly reduced and IL-10 significantly increased in all COVID-19 pneumonia patients with or without pre-existing COPD as a result of a daily dose of dexamethasone. Also, a significant reduction in cortisol, CRP, and LDH was observed following dexamethasone administration in all COVID-19 pneumonia patients, with or without preexisting COPD, with no correlation with gender, cigarette or waterpipe smoking, or opium abuse.ConclusionOur results showed a significant increase in TNF-α, IL-6, IL-10, cortisol, LDH, and CRP and a significant reduction in IL-10 in all critically ill COVID-19 pneumonia patients. Dexamethasone administration significantly reduced cortisol and proinflammatory cytokines and also LDH and CRP as the markers of COVID-19 severity.Keywords: COVID-19, COPD, Dexamethasone, Inflammatory Cytokines, Cortisol, C-Reactive Protein, Lactate Dehydrogenase
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Background
Microvascular changes and precursors of atherosclerosis are frequently observed in young patients with early-onset type 1 diabetes (T1DM). Due to their known beneficial effects, physical activity is strongly recommended for its management.
ObjectivesThis study aimed to investigate the effects of eight weeks of organized exercise (OE) and non-organized leisure time activity (NOLTA) on determinants of cardiovascular disease and physical fitness in children with type 1 diabetes.
MethodsIn this quasi-experimental study, 20 children with T1DM were randomly divided into two groups of 10: OE (n = 10, mean age = 12.60 ± 1.34 years) and NOLTA (n = 10, mean age = 12.40 ± 0.84 years). Subjects in both groups participated in their respective activities 3 days per week. Blood samples and physiological measurements were taken 48 hours before and after the 8-week training period.
ResultsHigh-density lipoprotein (HDL) levels and physical fitness factors increased significantly in both exercise groups. Fasting blood glucose, C-reactive protein, and the cholesterol/HDL ratio were also significantly reduced in both groups. Waist circumference, glycosylated hemoglobin, and the LDL/HDL ratio were significantly reduced only in the OE group. However, the decrease in the acute phase protein pentraxin 3 was minimal and statistically insignificant.
ConclusionsIn children and adolescents with T1DM, regular exercise—due to its anti-inflammatory effects and its ability to improve cardiorespiratory fitness, lipid profile, and blood glucose levels—may help reduce the severity and likelihood of cardiovascular disease in adulthood.
Keywords: Diabetes Mellitus, Type 1, C-Reactive Protein, Exercises, Pentraxin3, Inflammation, Lipid Profile -
Introduction
Hospital outcomes for myocardial infarction are among the clinical conditions influenced by the spread of COVID-19. Patients with COVID-19 frequently experience cardiovascular complications, with challenges encountered in acute management. We assessed clinical presentation, incidence, clinical outcomes and angiographic findings of myocardial infarction in COVID-19 patients.
MethodsThis study is a observational retrospective multicenter, medical diagram study was conducted on successive patients hospitalized with diagnosis of Covid-19 and myocardial infarction ,in two large referral hospitals with catheterization equipment and laboratories.COVID-19 infection was confirmed with reverse transcription–polymerase chain reaction assays of a nasopharyngeal sample or pattern of pulmonary parenchymal involvement in lung HRCT (approved by an expert respiratory or infection disease specialist). Data collected included patient demographics, comorbidities, electrocardiogram(ECG) and echocardiography results ,inpatient medication, treatment (fibrinolytic therapy, percutaneous coronary intervention (PCI) ,coronary artery bypass graft (CABG), vasopressor use, invasive mechanical ventilation),laboratory test results (leucocyte count, C-reactive protein , D dimer, BUN, Cr, and ferritin)and outcome(duration of hospitalization, revascularization success, in-hospital reinfarction and mortality).
ResultsThe most common comorbidities were hypertension (29, 58%), diabetes mellitus (21, 42%), dyslipidemia (14, 28%) and smoking (5, 10%). Fourteen patients (44.4%) were treated with PCI and 8 (19.5%) patients with fibrinolytic therapy as the initial reperfusion strategy. Revascularization was successful in 62% of patients. The median CRP level of patients died was 96, which was significantly more than the level (46) in discharged cases (p<0.001). Creatinine levels were also significantly higher in patients who died compared to those who were discharged (p=0.008).
ConclusionThe results of this study demonstrate upper mortality rate in patients with diabetes, kidney injury and high-level CRP, denoting the baseline clinical and laboratory data could be defined as prognostic markers in COVID-19 patients, especially while managing myocardial infarction with concurrent COVID-19 infection.
Keywords: Myocardial Infarction, Covid-19 Infection, Mortality, Creatinine, C-Reactive Protein -
Background & Objective
Subclinical hypothyroidism (SCH), is defined as an asymptomatic state characterized by a normal serum concentration of free thyroxine and elevated serum concentration of TSH. This study aims to investigate the complex interplay between hyperinsulinism, insulin resistance, beta cell function, and low-grade chronic inflammation in Iranian women with SCH.
MethodsEighty women with SCH and 80 healthy women as controls matched to the patient group for sex, age, and body mass index (BMI), were enrolled in this prospective cross-sectional study. TSH, free T3, free T4, highly sensitive C-reactive protein (hs-CRP), fasting insulin, fasting glucose, total cholesterol, HDL-cholesterol, LDL-cholesterol, Triglyceride, HOMA-IR index, Beta cell function (HOMA-B index) and insulin sensitivity were determined.
ResultsHs-CRP was not statistically different between the SCH patients and the control group (3.7±3.2 Vs 3.6±4.0, P>0.05). Total cholesterol and LDL-cholesterol were significantly higher; however, triglyceride and HDL-cholesterol were not statistically different in patients with SCH as compared with the control group. Fasting insulin levels and HOMA-IR and Beta cell function (HOMA-B) were significantly higher in SCH women compared to the control group. A positive correlation between HOMA-IR and HOMA-B with TSH levels was found (r=0.324, r=0.191, P<0.05 respectively). A positive correlation between insulin levels and hs-CRP (r=0.22, P<0.05), also between insulin levels and TSH (r=0.312, P<0.05) and LDL- LDL-cholesterol was obtained (r=0.27, P<0.05).
ConclusionIranian women with SCH may exhibit elevated atherogenic parameters (hyperinsulinemia, LDL-cholesterol, and total cholesterol), HOMA-IR, and HOMA-B.
Keywords: Beta Cell Function, C-Reactive Protein, Insulin Resistance, Subclinical Hypothyroidism -
BackgroundCurrent data indicate that serum vitamin D and susceptible C-reactive protein (hs-CRP) levels, both indicative of the inflammatory state, have the potential to predict the onset and severity of chronic pain. Therefore, the objective was to assess the intensity of pain experienced after breast cancer treatment and its relationship with these two parameters.MethodIn this cross-sectional study between 2019 and 2021, 201 patients were enrolled. The McGill Pain Questionnaire was employed to evaluate localized pain intensity at the site six months after the conclusion of cancer treatments. Patients were stratified based on the type of breast surgery, with or without a tissue expander, axillary region surgery, chemotherapy treatment, radiotherapy treatment, serum vitamin D levels, serum hs-CRP levels, and pain intensity. Data analysis was performed using SPSS 21 software with a significance level set at 0.05.ResultsAmong the patients, 67.6% (136 individuals) reported mild pain, 31.3% (63 individuals) reported moderate pain, and 1% (2 individuals) reported severe pain. The results of this study demonstrated a positive correlation between high serum hs-CRP levels and increased pain intensity, with serum marker levels being higher in patients experiencing more severe pain compared with those with milder pain. However, no statistically significant association was observed between various serum concentrations of vitamin D and pain intensity (P = 0.12).ConclusionElevated levels of inflammatory factors, such as hs-CRP, are linked to a higher likelihood of developing chronic post-surgical pain.Keywords: Breast Neoplasms, Pain, Vitamin D, C-Reactive Protein, Inflammation
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Background
Hypothyroidism is a significant contributor to complications in various metabolic and cardiovascular diseases. Thyroid hormones, including lipids, play a crucial role in macromolecule metabolism. C-reactive protein (CRP) is a key marker for cardiovascular diseases and hypothyroidism independently. This study aimed to evaluate serum high-sensitivity CRP (Hs-CRP) levels, lipid profiles, and their relationship with thyroid hormones in patients with hypothyroidism compared to a control group.
MethodsThis case-control study included 90 participants (45 hypothyroid patients and 45 healthy controls). Blood samples were collected, centrifuged to separate serum, and analyzed using commercial kits. ELISA measured T3, T4, TSH, and Hs-CRP, while TG, HDL, LDL, VLDL, and total cholesterol were measured by spectroscopy.
ResultsThe findings revealed higher Hs-CRP levels in hypothyroid patients compared to controls, although not statistically significant (P-value = 0.09). Hs-CRP positively correlated with TSH, T3, and TG but negatively correlated with T4, total cholesterol, VLDL, and HDL in hypothyroid subjects. These correlations were only significant between Hs-CRP and TG and Hs-CRP and LDL (P-value < 0.05).
ConclusionThe positive correlation between Hs-CRP and TSH in hypothyroidism highlights a potential link between hypothyroidism and cardiovascular disease.
Keywords: Hypothyroidism, Lipid Metabolism Disorders, C-Reactive Protein, Cardiovascular Disease -
Objective
This study assessed the effects of Aloe verasupplementation on serum inflammatory factors, blood sugar andlipid profiles in hemodialysis patients.
Materials and MethodsTotally, 50 hemodialysis patients wereallocated randomly to either Aloe vera or placebo groups. TheAloe vera group received 2 Aloe vera capsules daily for 8 weeks(500 mg/day). Serum C-reactive protein (hs- CRP), Fasting bloodglucose (FBS), and lipid profiles levels were evaluated at thebaseline and the end of the eighth week.
ResultsAloe vera supplementation for 8 weeks was associatedwith a significant reduction of serum hs- CRP (p=0.004), totalcholesterol (p=0.01), low density lipoprotein (LDL) (p=0.02) levesand increased high density lipoprotein (HDL) (p=0.002)concentration in the hemodialysis patients.
ConclusionAloe vera supplementation is beneficial inimprovement of cardiovascular risk factors in hemodialysispatients
Keywords: Aloe Vera, C-Reactive Protein, Inflammation, Lipids, Hemodialysis -
Journal of Research in Applied and Basic Medical Sciences, Volume:10 Issue: 3, Summer 2024, PP 233 -239Background & Aims
Mucormycosis, commonly known as "Black Fungus," is an angio-invasive fungus and has become a significant concern during the Coronavirus disease 2019 (COVID-19) pandemic. This study aimed to determine the prevalence rate of mucormycosis and related risk factors among COVID-19 patients, making an essential contribution to further understanding this opportunistic disease.
Methods & Materials:
This descriptive-analytical cross-sectional study was carried out among 276 patients. Patients were divided into two groups according to the presence of mucormycosis. The diagnosis of mucormycosis was established through a combination of clinical symptoms, imaging findings, and microbiological confirmation. The study examined demographic and clinical characteristics of the patients, including age, gender, duration of hospitalization, and laboratory results, such as C-reactive protein (CRP) and blood sugar levels. The statistical analysis was performed using the SPSS Statistics software package (version 26). Statistical significance was set at a P-value of 0.05.
ResultsThe mean age of patients participating in the study was 59±16 years. Among them, 158 patients (57.2%) were male, and 20 (7.2%) were diagnosed with mucormycosis. The analysis revealed a significant association between mucormycosis presence and various demographic and clinical variables, notably impacting hospitalization duration, blood sugar level, and serum CRP level.
ConclusionFindings from our study indicate that elevated CRP and blood sugar levels, as well as prolonged hospitalization, are significant risk factors for mucormycosis in the patients.
Keywords: Blood Sugar, C-Reactive Protein, Coronavirus Infection, Corticosteroids, Mucormycosis, Opportunistic Infection -
Background
Hemodialysis (HD) is the treatment of end-stage renal disease (ESRD), which leads to increased inflammation and mortality. This study aimed to investigate the relationship of neutrophil-to-lymphocyte ratio (NLR) and platelet-to-lymphocyte ratio (PLR) with high sensitivity C-reactive protein (hs-CRP) levels to predict inflammatory conditions in HD patients.
Materials and MethodsA total of 100 eligible maintenance HD patients referred to 22 Bahman and 17 Shahrivar hospitals in Mashhad were enrolled in this cross-sectional study. We collected demographic and clinical data, as well as values of inflammatory markers such as NLR, PLR, and hs-CRP, from the patient’s medical records.
ResultsForty-six of the patients were female. The mean age of the patients was 37.96±10.29 years, and the mean duration of HD was 54.32±50 months. The mean hs-CRP levels, NLR, and PLR were 6.08±5.70 mg/L, 2.79±1.18, and 125.80±57.14, respectively. The results showed a statistically significant and direct relationship between PLR and hs-CRP levels (P=0.01). There was no correlation between NLR and hs-CRP levels (P=0.8). Additionally, PLR was inversely correlated with hs-CRP in hypertensive patients (r=-0.283, P=0.04), female patients (r=0.3, P=0.04), and patients with HD duration of less than five years (r=0.3, P=0.001).
ConclusionBased on these findings, the PLR is a useful inflammatory marker in HD patients with ESRD that correlates with hs-CRP levels. We recommend further investigations to establish its accuracy in clinical care.
Keywords: Chronic Kidney Disease, C-Reactive Protein, Renal Dialysis, Neutrophil To Lymphocyte Ratio, Platelet To Lymphocyte Ratio -
سابقه و هدف
همه گیری کووید-19 ضمن ایجاد مشکلات بهداشتی- درمانی موجب مرگ و میر بیماران گردید. شناسایی فوری بیماران، بررسی پارامترهای ایمونولوژیک و هماتولوژیک و ارتباط آن ها با یکدیگر در تعیین روند درمان اهمیت دارد. لذا این پژوهش باهدف بررسی ارتباط شاخص های گلبول قرمز با CRP و IL-6 در بیماران مبتلا به کووید-19 انجام شده است.
مواد و روش هادر این مطالعه مقطعی توصیفی- تحلیلی، اطلاعات 280 پرونده مربوط به بیماران بستری مبتلا به کووید-19 بیمارستان های همدان در سال های 1399 و 1400 در نرم افزار SPSS نسخه 27 وارد گردید. داده ها با استفاده از آزمون مربع کای و ضریب همبستگی اسپیرمن و 05/0 p< تحلیل شد.
یافته هابا بررسی پرونده افراد مبتلا به کرونا مشخص شد که شاخص های Hb ، Hct ، MCHC ، RDW و MCV با ضریب همبستگی های 16/0 ، 19/0 ، 16/0، 21/0 و 23/0 با میزان IL-6 ارتباط معناداری دارند (به ترتیب 007/0 p= ، 002/0 p= ، 009/0 p= ، 001/0 p< و 001/0 p<). این در حالی است که هیچ یک از شاخص های مورد بررسی با میزان CRP ارتباط معناداری ندارند.
نتیجه گیریارزیابی دقیق شاخص های گلبول قرمز و ارتباط آن ها با پارامترهای ایمونولوژیک در انتخاب روش درمانی مناسب، پیش بینی شدت بیماری و تظاهرات بالینی، تعیین سطح خدمات مورد نیاز و تامین مراقبت های ویژه برای بیماران کمک کننده است.
کلید واژگان: کووید-19, گلبول های قرمز, شاخص های گلبول قرمز, CRPBackground and ObjectivesThe covid-19 epidemic leads to more mortality while creating health-treatment problems. Exact identification of the patients, evaluation of immunologic and hematologic parameters and their relationship is important in determining the treatment process. Therefore, this study was conducted with the aim of investigating the relationship between red blood cell indices and CRP and IL-6 in patients with covid-19.
Materials and MethodsIn this descriptive-analytical cross-sectional study, the data of 280 hospitalized patients with covid-19 in 3 Hamedan hospitals during 2020 and 2021 were entered in SPSS version 27 software. Data were analyzed using Chi square test, Spearman correlation coefficient, with the significance level (p< 0.05).
ResultsBy evaluation of the data of covid-19 patients, it was found that Hb, Hct, MCHC, RDW, and MCV indices with correlation coefficient of 0.16, 0.19, 0.16, 0.21, and 0.23 are related to IL-6 level significantly (p : 0.007, 0.002, 0.009, < 0.001, and < 0.001, respectively). Meanwhile, none of these indices have a significant relationship with the CRP level.
Conclusions :
Accurate assessment of red blood cell indices and their relationship with immunological parameters could be helpful in term of choosing proper treatment method, predicting disease severity and clinical manifestations, determining required service level, and providing intensive care for patients.
Keywords: COVID-19, Red Blood Cells, Red Cell Indices, C-Reactive Protein -
Background and Aim
Influenza mainly involves the pulmonary system, but may cause damage to other organs like liver, kidneys, and bone marrow. It is essential to identify changes in the laboratory markers indicating inflammation and hepatic injury; and changes in white blood cell count in patients with flu. This study aimed to evaluate these laboratory markers and compare them in children and adults.
MethodsThis retrospective study was performed in three university hospitals in Qazvin, Iran. Clinically available laboratory biomarkers including some inflammatory markers (e.g. CRP and ESR) , marker of hepatic injury (AST, ALT, ALP, billirubin, prothrombin time), and white blood cell count of 435 definite inflenza patients with positive influenza RT-PCR in 2018 and 2019 were collected from their medical records. The results were classified based on age (over 14 years of age in adults) and the normal range reported by the performing laboratory. Using SPSS software 25, data were analyzed by chi-square and pearson correlation coefficient test. P<0.05 was considered significant.
ResultsLeukocytosis was observed in 22.5% of adults and 1.0% of children, and the difference between the two groups was significant (p<0.001). ESR was increased in 91.6% of children and 86.9% of adults;CRP was elevated in 34.7% of adults while 80.5% of children showed elevation (p<0.001). The duration of hospital stay was significantly correlated with ESR (p<0.001, r= 0.177), and CRP (p= 0.002, r= 0.163).The increase in ALT was detected in 32.1% of adults versus 10.0% of children and AST in 38.6% of adults versus 10.0% of children. This increase in both liver enzymes was greater in adults compared with children (p<0.044 and p<0.013, respectively). 42.4% of adults, showed increased prothrombin time.
ConclusionLeukocytosis, CRP elevation and increase in liver enzymes during influenza seems to be more pronounced in adults than in children. The results also showed a significant relationship between a hospital stay and the level of ESR and CRP.
Keywords: Influenza, Liver Function Tests, Biomarkers, Blood Sedimentation, C-Reactive Protein -
Background
It has been known for centuries that plants and plant-derived chemicals are useful in treating diseases, with Aloe vera being one of the biologically active plants. A significant role is played by the C-reactive protein (CRP) in many inflammatory conditions, while leukocytes play an integral role in the response of the immune system to infectious diseases.
ObjectivesThis study explored the potential anti-inflammatory effects of Aloe vera methanolic extract on the total white blood cell (TWBC) count and CRP levels in Wistar rats received formalin.
MethodsA total of 25 Wistar rats were divided into five groups (A to E), with group A serving as the control and the other groups receiving formalin to induce inflammation, followed by increasing doses of Aloe vera extract. The CRP level was measured using an enzyme-linked immunosorbent assay (ELISA) kit, and the TWBC count was determined using a Sysmex haematology autoanalyzer.
ResultsResearch findings showed that 200 mg of Aloe vera methanolic extract effectively reduced the CRP level (0.056±0.0027) (P=0.04), while 600 mg of the extract significantly increased the TWBC count (13.920±4.757) (P=0.03).
ConclusionThese findings suggest that Aloe vera extract may be an effective natural supplement for improving the immune system function and reducing inflammation in the body. However, further research is needed to fully understand the underlying mechanisms for these effects and to determine the optimal doses and potential side effects.
Keywords: Aloe vera, C-reactive protein, Leukocytes, Immune system, Inflammation, ELISA -
Introduction
The use of Securidaca longipedunculata for solving health problems related to immunological disorders is long-standing. The immunomodulatory activity associated with the anti-inflammatory effect of S. longipedunculata has not yet been elucidated by scientific research. The aim of our study is to show that the leaves of S. longipedunculata can solve problems related to immunodeficiency and inflammation.
MethodsThe phytochemical compounds of the plant were carried out by solubility tests. The anti-inflammatory activity of the plant was evaluated by the chicken egg albumin denaturation inhibition, membrane stabilization, and C-reactive protein (CRP) tests. The 2,2-diphenyl1-picrylhydrazyl, total antioxidant capacity, and inhibition of lipoperoxidation tests were performed to evaluate the antioxidant activity of the plant. Immunosuppression was induced in rats by cyclophosphamide. The immunomodulatory activity of S. longipedunculata was studied by blood count. The lactate dehydrogenase (LDH) titration showed the effect of S. longipedunculata on the energy balance.
ResultsThe extract contained polyphenols, flavonoids, and tannins. The tests of albumin denaturation inhibition, membrane stabilisation, CRP, total antioxidant capacity, and 2,2-diphenyl-1-picrylhydrazyl revealed that the extract had anti-inflammatory and antioxidant effects. The blood count results revealed that the extract non-significantly increased the number of leukocytes except in the case of neutrophils and monocytes at a dose of 400 mg/kg (P<0.05). The extract also restored the energy balance in the rats according to the LDH results.
ConclusionThis study reveals that the hydro-ethanolic extract of S. longipedunculata leaves has potential immunostimulant and anti-inflammatory effects.
Keywords: Medicinal plant, Leukocytes, C-reactive protein, Oxidative stress, Cyclophosphamide -
مقدمه
بیماری مولتیپل اسکلروزیس (Multiple sclerosis) MS، یک بیماری پیش رونده ی التهابی می باشد که ماکرهای التهابی نقش موثری در گسترش این بیماری دارند. در مقابل، تمرین ورزشی ممکن است منجر به بهبود التهاب مزمن شود. با این وجود اثر تمرین ورزشی بر مارکرهای التهابی در این بیماران به وضوح درک نشده است. از این رو، هدف فراتحلیل حاضر بررسی تاثیر تمرین ورزشی بر سایتوکین های التهابی IL-6، TNF-α و IL-10 و همچنین CRP در بیماران مبتلا به MS می باشد.
روش هاجستجوی نظامند در 3 پایگاه اطلاعاتی اصلی PubMed، Web of Science و Scopus برای مقالات انگلیسی و فارسی منتشر شده تا تاریخ 1 دی 1401 انجام شد. به منظور تعیین اندازه ی اثر تمرین ورزشی در برابر گروه شاهد بر IL-6، IL-10، TNF-α و CRP از تفاوت میانگین استاندارد شده (SMD) و فاصله ی اطمینان 95 درصد (CIs) استفاده گردید.
یافته ها16 مطالعه شامل 687 بیمار مبتلا به MS وارد فراتحلیل شدند. تمرین ورزشی منجر به کاهش معنی دار سطوح IL-6 [0/002 = P، (0/14- الی 0/65CI: -) 0/40-] و CRP [0/02 = P، (0/04- الی 0/70CI: -) 0/370-] گردید، اما بر سطوح TNF-α [0/20 = P، (0/14 الی 0/68CI: -) 0/27-] و IL-10 [0/94 = P، (0/43 الی 0/40CI: -) 0/15] اثر معنی داری نداشت.
نتیجه گیریتمرین ورزشی ممکن است منجر به بهبود وضعیت التهاب مزمن به واسطه ی کاهش IL-6 و CRP در بیماران مبتلا به MS شود. از این رو، تجویز تمرینات ورزشی منظم برای بیماران MS ممکن است رویکرد مناسبی برای مدیریت بیماری باشد.
کلید واژگان: تمرین ورزشی, سایتوکین, التهاب, پروتئین واکنشی-C, اینترلوکین-6, اینترلوکین-10, فاکتور نکروزدهنده ی تومور-آلفا, مولتیپل اسکلروزیسBackgroundMultiple sclerosis (MS) is a progressive inflammatory disease in which inflammatory markers play an effective role in the development of this disease. In contrast, exercise training may lead to improved chronic inflammation. However, the effect of exercise training on inflammatory markers is not clearly understood. Therefore, the purpose of this meta-analysis is to investigate the effect of exercise training on IL-6, TNF-α, and IL-10 as well as CRP in patients with MS.
MethodsA comprehensive search was conducted in the three principal databases of PubMed, Web of Science, and Scopus for English and Persian articles published until December 22, 2022. To determine the effect sizes of the effect of exercise training on inflammatory markers IL-6, IL-10, TNF-α, and CRP, standardized mean difference (SMD) and 95% confidence intervals (CIs) were calculated.
FindingsSixteen studies involving 687 patients with MS were included in the meta-analysis. Exercise training resulted in a significant decrease in levels of IL-6 [P = 0.002, (CI: -0.14 to 0.65: -0.40)] and CRP [P = 0.02 -0.04 to 0.70 CI: -0.37], but did not lead a significant effect on the levels of TNF-α [P = 0.20, (0.14 to 0.68 CI: -) -0.27] and IL -10 [P = 0.94, (0.43 to 0.40 CI: -0.15)].
ConclusionExercise training may lead to improvement of chronic inflammation status by reducing IL-6 and CRP in patients with MS. Therefore, prescribing regular exercise may be a suitable approach for disease management in patients with MS.
Keywords: Exercise, Cytokines, Inflammation, C-Reactive Protein, Interleukin-6, Interleukin-10, Tumor Necrosis Factor-alpha, Multiple Sclerosis -
Introduction
The combined use of methadone and exercise training holds promise for promoting health. This study investigates the impact of resistance and aerobic training in conjunction with methadone consumption on lipid profiles and C-reactive protein (CRP) levels in addicted men.
Material & MethodsNinety addicted men (mean age 36.82 ± 4.32 years) undergoing prison treatment were randomly assigned to six groups (n=15 each): control, narcotics anonymous (NA), aerobic training + methadone, aerobic training + methadone reduction, resistance training + methadone, resistance training + methadone reduction. The 12-week exercise program, conducted thrice weekly, included aerobic training (4-8 sets, 3 minutes each, at 80-90% maximum heart rate on the ergometer bike) and resistance training (12 repetitions, 3 sets, at 70-85% of one repetition maximum). Blood samples collected pre- and post-intervention measured CRP and blood lipids using specialized kits. Data were analyzed with SPSS software, employing multiple analysis of variance (MANOVA) and Tukey's post hoc test (P<0.05).
ResultsHigh-density lipoprotein (HDL) levels significantly increased in trained groups compared to the control (P<0.001). Moreover, trained groups exhibited significant decreases in low-density lipoprotein (LDL), triglycerides, total cholesterol, and CRP compared to the control group (P<0.05). Importantly, aerobic training was more effective than resistance training in improving the studied variables.
ConclusionExercise training, particularly aerobic training with methadone ingestion, yields positive effects, including reduced CRP levels and improved lipid profiles in addicted men. Exercise training with methadone reduction may be considered for empowering individuals with addiction issues.
Keywords: Exercise, Lipids, C-reactive Protein, Methadone, Substance-Related Disorders -
Background
High-sensitivity C-reactive protein (hs-CRP), and homocysteine increase along with inflammation in type 2 diabetes mellitus (T2DM). The antioxidant properties of sumac might affect homocysteine and hs-CRP levels. We aimed to examine the effects of sumac powder (Rhus Coriaria L) on homocysteine and hs-CRP in patients with T2DM.
MethodsIn a single-blinded, randomized controlled clinical trial, conducted in Ardekan city, Yazd, Iran; 60 patients with T2DM were randomly divided into 2 groups to consume either low fat yogurt alone in the control group or along with 6 (2×3 grams) grams of sumac powder daily in the intervention group, for 3 months. Fasting blood samples were used to analyze the fasting blood sugar (FBS), serum homocysteine, and hs-CRP at the baseline and after 90 days. P values <0.05 were considered statistically significant.
ResultsFifty-eight individuals (intervention n=30, control n=28) with a mean age of 52.30±7.05 years in the intervention, and 51.61±7.07 years in the control group, finished the study. No significant differences were seen for FBS (p=0.94) and homocysteine (p=0.69) changes between the groups; but hs-CRP changes were significantly different between the groups (p=0.03).
ConclusionDaily consumption of sumac powder for 3 months may not have a reducing effect on FBS and serum homocysteine. This is while sumac may prevent increment of hs-CRP in patients with T2DM.
Keywords: Sumac, Rhus Coriaria, Homocysteine, C-reactive protein, diabetes mellitus -
زمینه و هدف
نشت آناستوموز یک عارضه ی جدی و شایع پس از Lower Anterior Resection (LAR) است که با افزایش میزان مرگ و میر بیماران و عوارض ناتوان کننده بعد از عمل جراحی مرتبط می باشد. تشخیص زودهنگام این عارضه می تواند مرگ و میر و کوموربیدیتی همراه را کاهش بدهد و در شروع زودهنگام درمان مناسب بسیار موثر باشد.
روش کار:
در این مطالعه مشاهده ای، توصیفی، تحلیلی، تمامی بیماران مبتلا به کانسر رکتوم که در بخش های جراحی کولورکتال بیمارستان امام خمینی (ره) تهران در سال 1400 تحت عمل جراحیLAR قرار گرفته اند (درصورت عدم ابتلا به نقص ایمنی یا سابقه جراحی های شکمی و عدم نقص در اطلاعات) وارد مطالعه شدند. اطلاعات بالینی و آزمایشگاهی مرتبط جمع آوری شدند و پس از ورود در نرم افزار SPSS، تحت آنالیزهای کای اسکوار، T مستقل و ROC قرار گرفتند.
یافته هامیانگین سنی بیماران، 29/14±86/55 سال و مورتالیتی 86/3% بوده است. بین نشت آناستوموز لگنی بعد از عمل جراحی LAR با سایرعوارض بعد از عمل جراحی و نیزIn-hospital mortality ارتباط معناداری یافت شد (05/0>P). میانگین سنی افرادی که لیک آناستوموز داشتند به طور معناداری از گروه دیگر بیشتر بود (05/0>P). میزان میانگین CRP در روزهای دوم، چهارم، و ششم، به طور معناداری در گروه دارای نشت آناستوموز از گروه دیگر بیشتر بوده است (05/05>P). توانایی بیشتر CRP روز ششم در تمایز دقیق موارد دارای لیک آناستوموز و گروه فاقد آن با AUC (area under the curve) برابر با 88/0 نشان داده شد.
نتیجه گیریسطح سرمی CRP به ویژه در روز ششم بعد از جراحی، می تواند یک فاکتور پیش گویی کننده مفید برای ارزیابی نشت آناستوموز در بیمارانی که تحت LAR قرار گرفته اند باشد که با مداخله درمانی مناسب نیز، امکان کاهش عوارض متعاقب آن وجود خواهد دارد.
کلید واژگان: Protein, نشت آناستوموز, کانسر رکتوم, Lower Anterior Resection, C-ReactiveBackground & AimsColorectal cancer is the third leading cause of cancer-related deaths globally. Despite advancements in chemotherapy and radiotherapy for rectal cancer, surgical resection remains the primary treatment. Among various surgical techniques, low anterior resection (LAR) is particularly common. However, the procedure’s complication rates are significant. Anastomotic leakage, a serious complication post-rectal resection, occurs in 1-19% of cases and can result in prolonged hospital stays, infections, permanent stoma formation in 25% of patients, and even mortality. Patients with anastomotic leakage often require surgical intervention to prevent death. In such cases, most surgeons choose anastomosis takedown with the Hartmann procedure, which is complex and carries significant risks. Consequently, many patients face permanent stomas, negatively impacting their quality of life. Therefore, preventing anastomotic leakage is crucial. Diagnosing anastomotic leakage is challenging and often delayed. Clinical assessments by surgeons have low predictive value for this complication, and there is frequently a lag between the onset of leakage and changes in vital signs or leukocyte counts. C-reactive protein (CRP), an acute phase serum protein, rises following rectal resection and may indicate postoperative infectious complications, including anastomotic leakage. This study aims to evaluate serum CRP levels post-LAR in rectal cancer patients, assessing its association with anastomotic leakage.
MethodsThis prospective cohort study included rectal cancer patients who underwent LAR surgery at Imam Khomeini Hospital in Tehran during 2021-2022. Based on inclusion and exclusion criteria, 66 patients were selected. Inclusion criteria involved patients undergoing LAR for rectal cancer, while exclusion criteria included immunodeficiency, previous abdominal surgeries, and incomplete data. Eligible patients were followed post-surgery, and demographic data (age, sex, BMI, diabetes history, smoking, distant metastases, neoadjuvant chemoradiotherapy history, TNM staging, leukocyte levels on postoperative days two, four, and six, complications, hospital stay length, and mortality rate) were collected. Serum CRP levels were assessed on postoperative days two, four, and six. Anastomotic leakage was identified based on clinical and radiological findings, and patients were categorized into leakage and non-leakage groups. The incidence of anastomotic leakage and its relationship with clinical/laboratory factors were statistically analyzed. The predictive value of postoperative serum CRP levels for early detection of anastomotic leakage was assessed using receiver operating characteristic (ROC) curves, and CRP levels were compared between the two groups across different postoperative days.
ResultsThe study included 66 patients (43 men, 23 women), aged 25-77. Anastomotic leakage occurred in 23.3% of men and 26.1% of women. Among the participants, 10.6% had diabetes and 16.7% smoked. Notably, 57.1% of diabetics and 27.3% of smokers experienced anastomotic leakage. 75.8% received neoadjuvant chemotherapy, and 80.3% had neoadjuvant radiotherapy, with leakage observed in 28% and 26.4%, respectively. 15.2% had distant metastases, with anastomotic leakage in 40% of these cases. Two patients (3.86%) died within 30 days post-surgery, both with a history of leakage and infection. Anastomotic leakage incidence by TMN stage was 33.3% for stage one, 23.5% for stage two, 41.2% for stage three, and 80% for stage four. Chi-square tests assessed the correlation between postoperative pelvic anastomotic leakage and factors like sex, diabetes history, smoking, neoadjuvant chemotherapy, neoadjuvant radiotherapy, postoperative complications, in-hospital mortality, and distant metastasis. Significant associations were found between leakage and postoperative complications and in-hospital mortality (p=0.000 and 0.001, respectively). No significant associations were observed for other factors. No significant correlation was found between leakage and TMN stage (p=0.148), but a significant association was observed between leakage and age groups (p=0.001). Independent t-tests comparing mean serum leukocyte levels indicated no statistical significance (p-values of 0.20, 0.07, and 0.06 on days two, four, and six, respectively). Mean CRP levels were 100.5 mg/L (leakage group) and 72.42 mg/L (non-leakage group) on day two, 105.68 mg/L and 75.92 mg/L on day four, and 153.12 mg/L and 103.16 mg/L on day six. Independent t-tests comparing CRP levels indicated significance (p-values of 0.0001, 0.0100, and 0.0000) across these days. ROC curve analysis established cut-off values of 98 mg/L for CRP on days two, four, and six, maximizing sensitivity and specificity for predicting leakage.
ConclusionIn our study, CRP levels on days two, four, and six were significantly higher in the leakage group. ROC analysis yielded cut-off values of 98 mg/L on postoperative days two, four, and six, demonstrating higher predictive power for leakage on day six. Previous studies also identified CRP as a predictor of anastomotic leakage. Ortega-Deballon et al. found CRP on days two and four as strong predictors of leakage and other septic complications, with day four showing areas under the curve of 0.804 and 0.787. Their cut-off of 125 mg/L on day four showed high sensitivity and negative predictive value for leakage. In our study, serum leukocyte levels in the leakage group exceeded those of the control group on all assessed days, though differences were not statistically significant. The consideration of WBC as a marker for leakage has been sparsely addressed, with some researchers reporting no significant correlation. Our findings revealed that 55.17% of patients with anastomotic leakage experienced other complications, and all patients with these complications also demonstrated leakage during hospitalization. Among those with leakage, 25% had diabetes, while 6% of the control group were diabetic, indicating a higher prevalence in the leakage group. Xiaoti Lin et al.’s meta-analysis linked diabetes independently with increased mortality risk due to leakage in colorectal surgeries, while Ziegler et al. found no impact on leakage but indicated higher mortality among diabetic patients with leakage. The average age of patients with leakage was significantly greater than that of the control group. Other studies also indicated that age correlates significantly with leakage risk. Lin et al. identified age over 70 as an independent risk factor for leakage.
Keywords: Anastomosis Leakage, C-Reactive Protein, Rectal Cancer, Lower Anterior Resection -
سابقه و هدف
آپاندیسیت حاد، یکی از دلایل شایع جراحی شکم، در هر رده سنی است و بیشترین موارد تشخیص نادر است که باعث جراحی غیرضروری (آپاندکتومی منفی) می شود. هدف از این مطالعه تعیین ارزش تشخیصی پروتئین واکنشی C (CRP) و اینترلوکین 6 (IL-6) در پیشگیری از آپاندکتومی منفی می باشد.
مواد و روش هااین مطالعه مقطعی بر روی 208 بیمار مراجعه کننده با شکم حاد جراحی و با تشخیص اولیه آپاندیسیت حاد در بیمارستان فاطمی اردبیل در مدت 6 ماه انجام شد. سطح پروتئین واکنشی C (CRP) و اینترلوکین 6 (IL-6) قبل از عمل جراحی در تمامی بیماران اندازه گیری شد. بیماران بر اساس نتایج پاتولوژی به دو گروه مثبت و منفی تقسیم شدند. سپس نتایج آزمایشگاهی با یافته های هیستوپاتولوژیک پس از جراحی مقایسه شد.
یافته هامیزان IL-6 در گروه مثبت 3/54±107/25 و در گروه منفی 2/41±75/50 بود که این اختلاف از نظر آماری معنی دار بود (0/032=p). میانگین CRP در گروه مثبت 12/1±35/5 و در گروه منفی 5/3±11/1 بود (0/001>p). بین IL-6 بیماران نرمال با IL-6 بیماران مثبت با جواب پاتولوژی چرکی (0/006=p) و گانگرن (0/001>p) ارتباط آماری معنی دار بود. بین IL-6 بیماران مثبت با جواب پاتولوژی هیپرپلازی فولیکولی با IL-6 بیماران مثبت با جواب پاتولوژی چرکی و گانگرن ارتباط معنی داری وجود داشت (0/001>p). همچنین بین IL-6 بیماران مثبت با جواب پاتولوژی التهابی با IL-6 بیماران مثبت گانگرن ارتباط آماری معنی دار بود (0/001>p). حساسیت، ویژگی، ارزش اخباری مثبت، ارزش اخباری منفی برای IL-6 به ترتیب 83، 88، 86، 79 و برای CRP به ترتیب 83، 85، 89، 81 بود.
نتیجه گیرینتایج مطالعه نشان داد که اندازه گیری همزمان CRP و IL-6 به عنوان بیومارکرهای مهم در تشخیص و پیشگیری از آپاندکتومی منفی مفید می باشند.
کلید واژگان: آپاندیسیت, اینترلوکین 6, پروتئین واکنشی C, آپاندکتومیBackground and ObjectiveAcute appendicitis is one of the common reasons for abdominal surgery in any age group, and most cases are rarely diagnosed, which causes unnecessary surgery (negative appendectomy). The aim of this study is to determine the diagnostic value of C-reactive protein (CRP) and interleukin-6 (IL-6) in the prevention of negative appendectomy.
MethodsThis cross-sectional study was conducted on 208 patients with acute abdominal surgery and primary diagnosis of acute appendicitis at Fatemi Hospital in Ardabil in a period of 6 months. C-reactive protein (CRP) and interleukin-6 (IL-6) levels were measured before surgery in all patients. Patients were divided into positive and negative groups based on pathology results. Then the laboratory results were compared with histopathological findings after surgery.
FindingsThe level of IL-6 in the positive group was 107.25±3.54 and in the negative group was 75.50±2.41, and the difference was statistically significant (p=0.032). The mean CRP was 35.5±12.1 in the positive group and 11.1±5.3 in the negative group (p<0.001). There was a statistically significant relationship between IL-6 in normal patients and IL-6 in positive patients with purulent (p=0.006) and gangrene (p<0.001) pathology. There was a significant relationship between IL-6 positive patients with follicular hyperplasia pathology and IL-6 positive patients with purulent and gangrene pathology (p<0.001). Moreover, there was a statistically significant relationship between IL-6 positive patients with inflammatory pathology response and IL-6 positive gangrene patients (p<0.001). Sensitivity, specificity, positive predictive value, negative predictive value for IL-6 were 83, 88, 86, 79 and for CRP were 83, 85, 89, 81, respectively.
ConclusionThe results of the study showed that the simultaneous measurement of CRP and IL-6 are useful biomarkers in the diagnosis and prevention of negative appendectomy.
Keywords: Appendicitis, Interleukin 6, C-Reactive Protein, Appendectomy
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