h. anbara

Sertraline is one of the antidepressants whose effects on the reproductive system has faced controversial reports. This study was performed to evaluate the efficacy of vitamin E against sertraline-induced injuries on serum and sperm parameters.

In this experimental study, 40 adult male mice were divided into eight groups of 5. Four groups of mice received vitamin E at 100 international units per kg body weight via gavage for 42 days. In three of the above groups, sertraline was administered orally at 5, 10 and 20 mg/kg body weight four hours after receiving vitamin E. One group was considered as the control group. Twenty-four hours after the final treatment, blood samples were collected from the heart and sperm quality parameters including count, motility, viability, chromatin density, abnormal forms, and sperm DNA damage were measured.

Sertraline significantly increased abnormal sperm (25.8±2.04) and significantly decreased the count (30.2±2.77), motility (68.30±2.94), viability (75.4±3.20) and maturation (84.0±1.58) of sperm compared to control group (10.4±1.14, 39.6±2.07, 89.37±1.87, 92.6±3.71, and 97.4±1.14, respectively) (P<0.05). Vitamin E administration significantly improved abnormal sperm (17.8±2.28), count (37.2±1.92), motility (78.27±2.10), viability (83.2±2.38) and maturation (83.4±1.81) of sperm (p<0.05).

The results of this study showed that vitamin E decreases the reproductive toxicity of sertraline in mice by improving serum and sperm parameters.

Anabolic steroids, especially dianabol, are used by athletes as a performance-enhancing drugs that damage the liver and cause structural changes. The aim of this study was to evaluate the effects of vitamin E on liver and kidney toxicity caused by dianabol. In this experimental study, 72 adult male mice were randomly divided into 8 groups of 9. Four groups of mice received 100 IU / kg vitamin E orally for 42 days through gavage. Three groups of the above groups received 5, 10 and 20 mg / kg oral dianabol four hours after receiving vitamin E, respectively. The control group and the groups receiving only 5, 10 and 20 mg / kg oral dianabol were also considered. 24 hours after the final treatment, serum samples were collected for biochemical evaluations and tissue samples were collected for histological, histomorphometric and histochemical evaluations. FINDINGS: The results showed that dianabol significantly increased the level of AST (158.52±9.76), ALT (113.70±11.02), and ALP (141.30±5.94), and significantly decreased albumin (1.04±0.47) compared to the control group (72.61±7.54, 41.47±7.03, 112.80±4.30, 3.14±0.25, respectively) (p<0.05). Administration of vitamin E significantly increased the level of AST (110.56±9.86), ALT (80.19±4.02) and ALP (120.52±4.94) and improved albumin (2.1±0.28) (p<0.05). The results of the study showed that vitamin E can reduce the oxidative damage caused by dianabol in the liver and kidney of the mouse.

In this study, the histology effect of caffeine administration was evaluated on retina and PAX6 expression in rat neonates. Material and Methods: A total of 24 pregnant rats were divided into three groups. Intraperitoneal injection of saline in the control group and also 50 mg/kg and 100 mg/kg caffeine injection from the ninth day of gestation was done. At 20 or 21 embryonic day, neonates on the first and fifth days were euthanized anesthetized and the eyes were isolated and fixed. The neonates were examined for abnormalities. The Retina Histological and morphometric evaluations, eye tissue fluorescence changes, and PAX6 gene expression were investigated by Real time PCR. The results showed that the effects of maternal caffeine treatment on the retina in neonates are associated with atrophic changes and cell counts of ganglion cell layer, retinal thickness and thickness of the inner plexiform layer, in the animals were treated with caffeine, were significantly reduced (P<0.05). Also caffeine induced behavioral changes in mothers and tissue fluorescence and significantly increased (P <0.05) the PAX6 gene expression in the groups receiving caffeine. Administration of caffeine during pregnancy can induce histopathological changes in organogenesis and developing in rat neonate’s retina.