mina aghazadeh

Ascites syndrome occurs in growing broilers in all parts of the world and is a major cause of losses in many flocks. In addition to genetic and non-genetic factors, differential expression of genes in susceptible chickens compared to resistant chickens during or before onset of the disease is likely involved. This difference in gene expression can be influenced by long non-coding RNAs. Therefore, the aim of this research was to identify and discover long non-coding genes effective in ascites syndrome in four tissues of broilers.Ascites.

Six data sets in four tissues (liver, kidney, heart and pulmonary artery) were used for this research. Hisat2 software was used to align the reads with chicken reference genome and Sting Tie software package was used to assemble the transcripts. To identify long non-coding genes, we used various software (CPC2, CNIT PLEK, FEELNC, PLIT) and BLAST methods. meta-RNAseq software and Fisher's method were used to perform meta-analysis.

Examination of the results of this research revealed that 188 lncRNAs were detected in the examined tissues and identified as new long non-coding RNAs. Of these, 160 lncRNAs were located within 50,000 contiguous 689 genes. Coexpression analysis showed that there was a correlation of 0.9 between 13 lncRNAs and 17 neighboring genes. Of these 17 neighboring genes, 6 coding genes were known. Examination of the level of significance between the healthy group and the ascites group in the examined tissues showed that in the pulmonary artery tissue the expression of 4 genes was significantly different and these 4 adjacent coding genes with 3 lncRNAs, i.e. H. an lncRNA that controls two adjacent genes. In the meta-analysis, 9 lncRNA genes were differentially expressed between healthy chickens and ascites chickens.

The results of this research showed that lncRNAs play an important role in theoccurrence of ascites disease and that genetic management can reduce the occurrence of thiscomplication in poultry farms and control economic losses.

 This study assessed anxiety and depression scores based on the Hospital Anxiety and Depression Scale (HADS) questionnaire, inpatients recalled after mammography.

 In this cohort study, women referred for mammography completed the HADS questionnaire. In 2019, 84 patients who required recall based on the results of their previous mammogram were selected and then completed the HADS questionnaire for the second stage. The HADS questionnaire was used to examine psychological problems. SPSS was used to analyze the collected data.

 In this study, 86 women who were screened with mammography were evaluated. The mean age was 47.2±8.3 years. Based on the HADS questionnaire, the mean anxiety score before mammography was 10.2±4.8, and after the recall was 13.2±4.2, which was statistically significant (P<0.001). Also, the depression score in these patients before mammography was 10.7±3.8, and after the recall was 12.6±3.6 (P<0.001).

 Recalling patients after mammography can increase their anxiety and depression, which can decrease patients’ desire for follow-ups.