yusuf a. j
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Objective
Thus, in this work, phytochemical screening, acute toxicity and antifungal evaluation on the methanol extracts of different parts (leaves, seed and aerial part) of Mitracarpus hirtus against Candida albicans, Candida tropicalis, Aspergillus fumigatus, Aspergillus niger, Histoplasm capsulatum, Mucor sp, Microsporum canis, Microsporum gallinae, Trichophyton mentagrophyte, and Trichophyton rubrum using agar well diffusion and broth dilution techniques.
Material and MethodsThe phytochemical screening of the methanol extracts of different parts of the plant revealed the presence of tannins saponins, flavonoids, alkaloids, steroids and terpenoids and the median lethal dose (LD50) was estimated to be greater than 5000 mg/kg which indicates that the plant is non-toxic.
ResultsSusceptibility test for the methanol extracts showed mean zone of inhibition ranging from 19.33±0.58-23.33±0.56 mm against the test organisms while the standard drug, Fulcin had 24.33±0.58 – 31.67±0.58 mm. The minimum inhibitory concentration (MIC) of the extracts ranges between 0.25-0.50 mg/mL while the minimum fungicidal concentration (MFC) ranges between 0.25-1.00 mg/mL against some of the fungal pathogens.
ConclusionThe findings of this study have revealed the antifungal efficacy of methanol extracts of the different part of M. hirtus which supports the ethno-medicinal claim of the use of the plant in the treatment of fungal infections.
Keywords: Mitracarpus hirtus, Secondary metabolites, Antifungal, Toxicity -
Objective
Tapinanthus globiferus is a hemi-parasitic plant used in ethonomedicine to treat different ailment including fungal infections. The aim of the study was to isolation the bioactive compound from leaf of T.globiferus growing on Balanites aegyptiaca.
Material and MethodsThe plant material was collected, identified pulverized to powder using mortar and pestle. The powdered plant material was subjected to maceration with 90% methanol to obtained crude methanol leaf extract which was further partitioned with solvent of increasing polarity to obtained n-hexane, chloroform, ethylacetate and n-butanol fractions.
ResultsChromatographic separation of ethylacetate fraction using a combination of silica gel column and sephadex LH-20 column led to the isolation of flavonoid (3,5,7- trihydroxy-2-(4-hydroxyphenyl)-4H-chromen-4-one). The structure of the compound obtained was established by Spectroscopic analysis (FTIR, NMR).
ConclusionChromatographic studies of ethyl acetate fraction of T. globiferus growing on Balanites aegyptiaca, afforded a 3,5,7-trihydroxy-2-(4-hydroxyphenyl)-4H-chromen-4-one.To the best of our knowledge, this is the first report of isolation of this compound from Tapinanthus globiferus growing on Balanites aegyptiaca.
Keywords: Isolation, Balanite aegyptiaca, Tapinanthus globiferus, NMR, FTIR
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