جستجوی مقالات مرتبط با کلیدواژه « Gene expression » در نشریات گروه « ریاضی »

Nowadays, given the numerous disease problems that have arisen in shrimp farms, the utilization of nutritional supplements to strengthen the immune response and controlling the common diseases in dense shrimp farming have been highly regarded.  So, the present study aimed to investigate the effect of basic diet formulated with different levels of Moringa oleifera leaf powder (25, 50 and 100g/kg) and ethanolic extract (0.25, 0.5 and 1g/kg) in seven treatments each with three replicates on the expression of immune genes in the Pacific white shrimp Litopenaeus vannamei. After 8 weeks, the mRNA expression of four immune genes, including alpha-2-macroglobulin (α2M), Lipopolysaccharide and B1,3-glucan binding protein (LGBP), Peroxinectin (PX) and integrinß, were measured in the hepatopancreas using Real-time PCR technique. The results showed a statistically significant differences in the expression levels of the α2M, LGBP, PX genes in all treatments (p<0.05). The expression level of α2M in treatments of 0.25 and 0.5 g/kg extracts and 25 g/kg Moringa leaf powder were significantly higher than that of the other treatments (p<0.05). The highest levels of LGBP expression were observed in 0.5 and 1g/kg Moringa leaf extract and the highest level of PX gene expression was observed in 0.5 and 1g/kg Moringa leaf extract and 25g/kg Moringa leaf powder treatments, which was significantly higher than other treatments and the control treatment (p<0.05). In conclusion, the results of this study showed the positive effects of using 0.5 and 1 g/kg Moringa leaf extract and 25 g/kg leaf powder on the functioning of the immune system in L. vannamei.

The present study was conducted to evaluate the effects of different levels of dietary protein and lipid on the expression of genes involved in immunity (lysozyme and TNF- 1α) and appetite (Ghrelin) in zebrafish, Danio rerio. So that, 9 diets with 3 protein levels (25, 30 and 35%) and 3 lipid levels (4, 8 and 12%) were prepared. One hundred and thirty five fish, 5 in each group, with an average weight of 1.23 ± 0.02 (g) were randomly distributed into 27 aquaria (20-liter) and fed 3 times daily during 4 months. A factorial experimental design was used for this study. The results revealed that the growth parameters (final weight, body weight gain, body weight gain percentage, specific growth rate and feed conversion ratio) and expression of immune and appetite genes increased significantly by increasing dietary protein levels in the treatment fed with 35% protein (p<0.05(. Increasing dietary lipid increased lysozyme expression and growth indices in fish fed with 12% lipid compared to other treatments; increased ghrelin gene expression in 12% compared to 4% significantly (p<0.05) and also insignificant increased TNF-1α expression (p>0.05). The combined effects of protein and lipid increased the lysozyme, TNF- 1α and ghrelin gene expression (p<0.05). The highest gene expression among all genes was observed in the diets containing 35% protein and 12% lipid. These findings revealed that dietary protein and lipid can enhance the function of immune system and appetite in zebrafish.