جستجوی مقالات مرتبط با کلیدواژه « Calcium sensor » در نشریات گروه « بیوتکنولوژی و ژنتیک گیاهی »

Calcineurin B-like proteins (CBLs) are a subfamily of calcium sensors that play a role in various plant cell processes and molecular functions. In sesame (Sesamum indicum), in silico analysis of the CBL gene family was performed to identify CBL proteins involved in calcium signaling. Using their orthologic relationships with Arabidopsis homolog genes, the nine SiCBL genes were identified and subdivided into six groups: SiCBL1, SiCBL2, SiCBL3, SiCBL4, SiCBL8, SiCBL10. The molecular weight of SiCBL proteins ranged from 24.4 to 37.9 kDa, the Isoelectric acid pH range, the instability index ranged from 33.99 to 47.46 percent, the aliphatic index ranged from 80.29 to 10.89, and the GRAVY ranged from -0.420 to 0.061. Prediction of post-translational modifications revealed that palmitoylation motif was observed in all siCBL, however majority of them did not have myristoylaton motif. In term of gene structure, 11% of SiCBL genes had nine exons, 11% had eight exons and 77% had seven exons. The RNA-seq pattern of the SiCBL subfamily under PEG treatment revealed that, whereas members of this gene family had generally similar expression patterns in both susceptible and tolerant cultivars, due to functional Convergence, each member of this gene family had a distinct expression pattern. Future research on the expression of SiCBL and SiCIPK gene family genes under various abiotic conditions could aid in understanding the mechanism of expression control of SOS-related genes.

Calcineurin B-like proteins (CBLs), which act as a secondary messenger molecule in the subfamily of the calcium sensor gene family, play a key role in regulating physiological processes, plant growth and development. In order to identification and comparison of proteins involving in calcium signaling in two model of halophyte and glycophyte plants, in silico analysis of the CBL gene family were done in Aeluropus littoralis and Arabidopsis thaliana. Based on sequence homology and orthological relationships with Arabidopsis genes, 6 genes identified in Aeluropus were classified into three protein groups: AlCBL4, AlCBL2 and AlCBL10. Multiple sequence allignment of the CBL gene family in Aeluropus littoralis confirmed the presence of four EF-hand domains in all genes, which provide a structure for calcium ion binding. The high similarity of the physicochemical properties of most Aeluropus proteins to Arabidopsis as well as the strong orthological relationship with each other may indicate the preservation of the function of these genes in the evolutionary process. Analysis of AtCBLs expression patterns in different organs/ abiotic stresses showed that these genes have unique expression profiles due to functional and structural convergent. Different expression profiles of AlCBLs in Aeluropus transcriptome would be an evidence for the functional divergent of these genes. The results obtained from this study can provide valuable information about the properties of this gene family and their functional roles in tolerating to abiotic stresses for future studies.