nano selenium
در نشریات گروه دامپزشکی-
Newcastle disease (ND) and Avian influenza (AI) are the major problems and the most economically important viral diseases in the poultry industry; therefore, vaccination against these diseases is considered one of the most effective ways of prevention. Extensive studies have been conducted to improve the performance of vaccines, and one of the major achievements of these studies is the preparation of adjuvants as stimulants of the immune system and one of the most important compounds in killed vaccines. An immunogenicity comparison of three adjuvants including, ISA70VG, Nano-Aluminum Hydroxide (Nano-Alum), and MF59 alone or with Nano-Selenium (Nano-Se), was performed using bivalent Newcastle plus Avian Influenza (ND+AI) killed vaccine. In this study, 105 specific-pathogen-free chicks (Ross-308) were divided into 7 treatments, including T1 (control group), T2 (ISA70VG), T3 (ISA70VG plus Nano-Se), T4 (Nano-Alum Hydroxide), T5 (Nano-Alum+Nano-Se), T6 (MF59), and T7 (MF59+Nano-Se). The vaccine was injected subcutaneously on day 21 in the back of the neck area. The blood samples were taken on days 14, 21, 28, 35, 42, and 49 post-vaccination. Serums of the samples were titrated by the haemagglutination inhibition (HI) test against Newcastle and Avian influenza. Based on the results, the highest HI test titers were observed for the T2 and T3 treatments, while the T6 and T7 treatments had the lowest titers. Moreover, regardless of the type of the adjuvants, adding Nano-Se increased the antibody titer in the vaccinated groups. In conclusion, a combination of the ISA70VG adjuvant and Nano-Se induced excellent antibody titers using bivalent ND+AI killed vaccine.
Keywords: Newcastle disease, Avian Influenza, Adjuvant, Nano-Selenium, MF59, ISA 70 VG, Nano-Aluminum Hydroxide -
It is well documented that aging has negative effects on fertility. With increasing age, the activity of antioxidant enzymes are reduced and because of roosters sperm composition, a high proportion of polyunsaturated fatty acids (PUFAs), the probability of sperm damage increases. The objective of the present study was to compare the effects of nano-selenium and sodium selenite on fertility in aged male broiler breeder chickens. Thirty-five male broiler breeders (Cobb 500)® at 50 weeks of age were randomly divided into five equal groups: The control group was fed on a commercial diet, group T1 was fed on a commercial diet supplemented with sodium selenite (0.30 mg kg-1 feed), group T2, T3 and T4 were fed on a commercial diet supplemented with nano-selenium (0.15, 0.30 and 0.60 mg kg-1 feed, respectively). Sperm characteristics (sperm count, motility, viability, and maturity) as well as testicular histomorphometric features [tubule differentiation (TDI), spermiation (SPI), Sertoli cell (SCI) and miotic (MI) indices] were assessed. The results showed that sperm characteristics were gradually decreased with age in the control group, however, it increased in group T3. Also, TDI, SPI, SCI, and MI in group T3 were higher than those of other groups. Our findings revealed that dietary supplementations with nano-selenium boosted fertility in aged male broiler breeders and the best results were obtained when the roosters received 0.30 mg kg-1 nano-selenium. Supplementation of nano-selenium in aged broiler breeder males might be effective to maintain flock fertility and/or increase the flock fertility.Keywords: Fertility, Histomorphometry, Male broiler breeder, Nano-selenium, Testis
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سلنیوم یکی از عناصر کم نیاز است که نقش بسیار مهمی را در بدن موجودات زنده بازی می کند و به عنوان یک آنتی اکسیدان در بدن مطرح است. نقش بیولوژیکی سلنیوم بر اساس تاثیر آن در ساختمان بسیاری از سلنوپروتین ها است. هدف از انجام این آزمایش بررسی اثر افزودن مکمل تغذیه ای نانو سلنیوم بر فعالیت آنزیم گلوتاتیون پراکسیداز در پلاسمای خون و منی قوچ عربی خوزستان بود. در این آزمایش از 12 راس قوچ نژاد عربی با میانگین وزنی 3±73 کیلوگرم و سن دو تا چهار سال در سه تیمار با چهار قوچ در هر تیمار مورد استفاده قرار گرفت که شامل گروه شاهد (بدون نانو سلنیوم) و دو گروه آزمایشی بودند که به ترتیب 4/0 و 8/0 میلی گرم نانوسلنیوم در هر کیلوگرم ماده خشک مصرفی دریافت کردند. نتایج نشان داد که در تیمار 4/0، غلظت سلنیوم خون و منی نسبت به شاهد افزایش یافت که این افزایش فقط در مورد خون معنی دار بود. در تیمار 8/0، غلظت سلنیوم خون و منی با غلظت آن در تیمار 4/0 و گروه شاهد به صورت معنی داری بالاتر بود. فعالیت آنزیم گلوتاتیون پراکسیداز خون و منی در تیمار 4/0 نسبت به گروه شاهد و در تیمار 8/0 نسبت به تیمار 4/0 و شاهد به طور قابل توجهی افزایش پیدا کرد. در نهایت این نتیجه حاصل شد که مکمل سازی نانو سلنیوم باعث افزایش فعالیت آنزیم گلوتاتیون پراکسیداز در خون و منی قوچ های عربی می شود و از نظر فعالیت این آنزیم تیمار 8/0 در خون و منی بهترین نتیجه را نشان داد.کلید واژگان: گلوتاتیون پراکسیداز، نانو سلنیوم، خون، منی، قوچ عربیSelenium is an essential nutrient that play a very important role in the body and is an antioxidant. The biological role of selenium is based on its effects on the structure of many selenoproteins. This experiment aimed to investigate the effect of nano-selenium nutritional supplementation on blood and semen glutathione peroxidase activity in Khuzestan Arabian rams. In this experiment, twelve Arabian rams with an average weight of 73±3 kg and two to four years old were used. Animals were divided into three groups; the control group (without nano-Selenium) and two experimental groups that received the 0.4 and 0.8 mg nano-selenium per kg dry matter, respectively. The results showed that selenium concentrations in blood and semen increased in treatment 0.4 compared to the control, which was significantly increased in the blood. Blood and semen selenium concentrations in group 0.8 were significantly higher than group 0.4 and control. The blood and semen glutathione peroxidase activity significantly increased in treatment 0.4 compared to control and in treatment 0.8 compared to the control and treatment 0.4. Finally, it was concluded that Nano-selenium supplementation increases the blood and semen glutathione peroxidase activity of Arabian rams and treatment 0.8 showed the best results.Keywords: Glutathione peroxidase, Nano-Selenium, Blood, Semen, Arabian ram
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Fertility is one of the most important parameters in breeder farms and cockerels play an outstanding role in fertility of eggs in broiler breeder units. Todays, supplementation of feed-additives such as organic selenium is used to increase fertility. The aim of this study was to evaluate the effects of different levels of Nano-Selenium (Nano-Se) on expression of molecular markers of spermatogonial stem cells (SSCs) in testis of broiler breeder males. A total of 30 roosters of 40 weeks age were randomly divided into 5 groups. Groups were as follow: 1) control (normal diet), 2) diet supplemented with 0.3 mg/kg sodium Selenite, 3) diet supplemented with 0.15 mg/kg Nano-Se, 4) diet supplemented with 0.3 mg/kg Nano-Se and 5) diet supplemented with 0.6 mg/kg Nano-Se. At the end of experimental period, birds autopsied and samples from testis of all birds were taken. The samples were used to examine the β1-integrin (CD29), thy-1(CD90 and NANOG mRNA expression by quantitative Real-Time PCR. The results of this study showed that testis of the groups fed with diets supplemented with 0.6mg/kg and 0.15mg/kg of Nano-Se had the highest and lowest mRNA expression of SSCs markers, respectively. In conclusion, the present study indicated that Nano-Se had advantages to sodium Selenite and 0.6 mg/kg of Nano-Se supplemented in males' diet in broiler breeders farms may contributes to optimal fertility via increasing mRNA expression of SSCs markers of roosters' testis and could be used to delay reduction of fertility caused by aging in broiler breeder males.Keywords: Nano-selenium, Molecular markers, Spermatogonial stem cells, Broiler breeder males
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A total of 192 one-day old Ross-308 broiler chickens were divided to 4 treatment groups of 48 chicks. Treatment groups were Control (Basal diet), vit-c (Basal diet+500mg/kg vit-c), Nano-Selenium (Basal diet+0.3mg/kg Nano-Se), and organic Selenium (Basal diet+0.3mg/kg Organic-Se). Chicks were raised until 35th day and growth performance was evaluated during the growing period. At day 35, 12 birds were selected in each group, bled and sacrificed to assess the relative weight of carcass, liver and spleen and to calculate the duodenal morphology and serum lipogenesis factors.Results showed that FCR of Nano-Selenium group was numerically better among groups but without significant differences (P>0.05) and relative carcass weight were also numerically higher in both vit-c and Nano-Selenium groups than that of others. The highest percent of liver weight were recorded in control group and had significant difference with vit-c group which could decrease relative liver weight (P<0.05). Moreover, spleen weight was increased in both Nano and organic Se groups. About morphological parameters of intestine, liberkuhn gland depth and villi height and surface of duodenum were enhanced in Nano-Selenium more than others and had significant differences with control group in all of the parameters (P<0.05). The differences of serum lipogenesis factors’ were not statistically significant but organic selenium had lower triglyceride and cholesterol levels than others. Meanwhile, PCV was decreased in Nano-Selenium groups. It could be concluded that Nano-Selenium is a better choice for improving performance parameters in broiler chickens than organic selenium and vit-c due to better impact on intestinal villi surface area and liberkun glands
Keywords: Nano selenium, Organic selenium, Vitamin C, Broiler chicken, Intestinal villi, Lipogenesis
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