جستجوی مقالات مرتبط با کلیدواژه « Garden Thyme » در نشریات گروه « دامپزشکی »

In order to apply the use of plant essential oils in complementary medicine, it is necessary to investigate their effects in inhibiting the growth of pathogenic bacteria in the laboratory, animal model and also consider their possible side effects. Therefore, the purpose of the present study was to investigate the antibacterial effects of garden thyme essential oil on Salmonella typhimurium and to evaluate its ability to prevent possible side effects caused by experimental exposure to the mentioned bacterium. For this purpose, 35 fourteen-day-old male broiler chickens were divided into 5 groups of 7 including healthy control, gavage control, experimental patient and experimental patient treated with garden thyme essential oil or Oxytetracycline. On the first day of the experimental period, 0.5 ml of Salmonella typhimurium microbial suspension and on subsequent days, 1 ml of garden thyme essential oil or Oxytetracycline was gavaged to all the chickens of the third to fifth groups and also 1 ml of sterile distilled water was gavage to the chickens of the second group twice a day with a time interval of 12 hours for 7 days. At the end of each stage, fecal microbial count was performed and at the end of the experimental period, all birds were euthanized for pathological examination of the small intestine and liver samples. The findings indicated that fecal bacterial shedding was observed throughout the experimental period in group 3 while in groups 4 and 5, fecal bacterial shedding was observed for 4 and 2 days after gavage respectively. The results demonstrated that Oxytetracycline treated chickens had a significant decrease in the excretion and colonization of Salmonella typhimurium.

Salmonellosis is one of the most important common diseases of livestock and humans worldwide. With the prevalence of antibiotic resistance, the tendency to use medicinal plants to control microorganisms has increased. The aim of this research was to investigate the antibacterial effects of the essential oils of Thymus vulgaris and salmonella typhimurium (ATCC=14028). For this purpose, first, the essential oils of the mentioned plants were extracted by steam distillation using a Clovenger machine. Then, using the microdilution broth method, the Minimum Inhibitory Concentration (MIC) and the Minimum Bactericidal Concentration (MBC) of essential oils alone and in combination, as well as drug sensitivity or resistance testing based on the well diffusion method in agar against the standard strain of Salmonella typhimurium bacteria, were determined in comparison with three common antibiotics in veterinary medicine. The MIC was calculated for thyme (0.25), mixed essential oil (0.50), doxycycline (0.0078) and oxytetracycline (<0.00195) μg/ml. The MBC for them was 0.25, 0.50, 0.0156 and >0.00195 respectively. Dill essential oil and erythromycin antibiotic had no antimicrobial effect against Salmonella typhimurium. The highest effect was related to oxytetracycline (with a growth inhibition diameter of 27 mm) and the lowest effect was related to thyme essential oil (with a growth inhibition diameter of 10 mm). Unlike dill essential oil, garden thyme essential oil has a significant antibacterial effect.