Floral dip Transformation of EXPA1 to Arabidopsis thaliana

Drought is by far the most important environmental stress in agriculture and many efforts have been made to improve crop productivity under water-limiting conditions.Minimizing the ‘yield gap’ and increasing yield stability under different stress conditions are of strategic importance in guaranteeing food for the future. Expansin is a protein super family with 4 families in vascular plants. Expansions are cell wall proteins which mediate acidic cell wall loosening through breaking hydrogen bonds between cellulose and glycan matrix. In this study pBIEXPA1 construct was constructed. Arabidopsis plants were transformed by pBIEXPA1 which contained nptII gene and AtEXPA1 gene which was under the control of CaMV35s promoter. Transformation was done by floral dip which did not need any tissue culture and transformed plants were T1. Transformed seedlings were able to remain green on MS medium containing 50 mg/L kanamycin. Transgenic plants were confirmed by PCR. As expected two 753 and 1080bp band were seen in transgenic plants.