Detection of Naturally Infected Vector Ticks (Acari: Ixodidae) by Different Species of Babesia and Theileria Agents from Three Differ-ent Enzootic Parts of Iran

Diagnostic study of vector ticks for different pathogens transmitted specifically have been done by Iranian old scientists working on the basis of biological transmission of pathogens. In this study we decided to con­firm natural infection of different collected ticks from three different provinces of Iran. Ticks were collected from livestock (sheep, goats and cattle) during favorable seasons (April to September 2007 and 2008). Slide preparations were stained by Giemsa and Feulgen and were studied searching for any trace of infection. Positive DNA from infected blood or tissue samples was provided and was used as positive control. First, PCR optimization for positive DNA was done, and then tick samples were subjected to specific PCR. Eleven pairs of primers were designed for detection of Theileria, Babesia and Anaplasma spp. Totally 21 tick samples were detected to be infected with protozoa. Hyalomma anatolicum anatolicum and Rhipicephalus turanicus from Fars Province were infected with T. lestoquardi at two different places. Hyalomma detritum was in­fected with T. lestoquardi in Lorestan Province and Rh. turanicus was infected to Ba. ovis from Fars Province. Totally 21 tick samples were detected to be infected with protozoa. Every sample is regarded with host-environment related factors. Since there are complex relations of vectors and their relevant protozoa, different proce­dures are presented for future studies.