Evaluation of bioactivity poly-3-hydroxybutyrate coated Nano-Bioglass 45S5 composite scaffolds for bone tissue engineering

Development of composite scaffolds due to combing useful properties of two or more materials in order to meet mechanically and physiologically host tissue is increasing. Firstly، nano-Bio glass (nBG) powder was made with grain size between 55-65nm and then porous nBG of 30، 40 and 50%wt was produced by polyurethane sponge replication method. In order to increase the scaffoldsmechanical properties، they coated with 6%wt poly-Hydroxybutyrate (P3HB) for 30sec and 1min. XRD،XRF، SEM، FE-SEM and FT-IR were used in order to study the phase and elemental structure، morphology، grain size and functional groups، respectively. The scaffold including 30%wt nBG and 6%wt was used for evaluation of bioactivity. Biocompatibility of the scaffold was also tested in SBF solution for 4 weeks at 37 ºC. XRD، SEM and AAS results showed formation of HA on the surface of scaffold which prove the its biocompatibility، the result is showing that، the best scaffold is 30%wt nBG /6%wt P3HB with 75-87% porosity، which is a good candidate for bone tissue engineering.