Study of Reference Genes in Sesame Leaves under Salt Stress by Real-Time PCR Method

Analysis of gene expression is considered as an essential part of functional genomics studies in all living organisms. Real-time PCR technique is very strong one to study the expression of a gene. However، despite its reliability، it has a set of specific problems، such as internal control gene selection which are suitable for normalization of the data. The study about selection reference of genes in sesame plant، at different developmental stages and under salinity stress of were studied. For this purpose، four internal control genes consists of eIF4- A، UBQ5، Alpha-Tubulin and Beta-Actin which are commonly used as housekeeping genes in plants، are selected and the stability of its expression in different salinity levels (zero and 75 mM) and different growth stages in five time periods (0 h، 6 h، 1 day، 4 days، 8 days and 16 days) in leaf tissue were examined. Study of the expression of reference genes using geNORM software showed that، in developmental stages and salinity in the leaf tissues، eIF4-A and Beta-Actin genes have more stable expression than other investigated genes. Using these genes can be useful in normalization of gene expression by Real-Time PCR analysis. The results can be used as reference genes for gene expression analysis in the Real-Time PCR.