Embryonic development and growth performances of an endangered fish species Nandus nandus: effects of dietary polyunsaturated fatty acids supplementation

The present study explored the embryonic and larval development of an endangered fish species Nandus nandus and resolved larval growth performances with the dietary supplementation of different types of lipids. Fertilized eggs were collected from fiber glass tanks immediately after spontaneous spawning of N. nandus, which were fed with a 1% phospholipid (squid meal) supplemented diet for 3 months. Fertilized eggs were transparent, spherical, yellowish and sticky in nature. The first cleavages of eggs were observed 0.3±0.01 h post fertilization at 26oC water temperature. Hatching started around 18 h post - fertilization and newly hatched larvae were found to be 1.2±0.1 mm in length. First feeding started 64.0±0.30 h post hatching. After rearing for 10 days, they were divided into 4 groups and separately fed with only dry tubificid worms, 1% docosahexaenoic (DHA) supplemented with dry tubificid worm, 1% phospholipid supplemented with dry tubificid worm and live tubificid worms as treatment I, treatment II, treatment III and treatment IV, respectively. After 50 days of the trial, larvae of treatment II showed significantly (p