Improvement of rainbow trout (Oncorhynchus mykiss) roe quality using chitosan and garlic and lemon extract

Different kinds of anesthetics decrease fish stress by affecting on fishes audaciousness and physiological side effects of stress in fish. In this study the effects of anaesthesia with MS222, Clove oil (Eugenia caryophyllata) and 2-Phenoxy ethanol on some haematological and immunological parameters of grass carp (Ctenopharyngodon idella) were investigated. Firstly the effective dose of each anaesthetics were calculated in grass carp. Secendly the effective dose of MS22, 2Phenoxy ethanol and Clove oil were determined as: 100, 200 and 100 mg/l, respectively. Fish were anaesthetized with the effective dose of each anaesthetic then blood samples were taken at 1, 12, 24 and 72 hours after anaesthesia. Haematologcal parameters (Packed cell Volume, Hemoglobin, Red blood cell count, White blood cell count, differentiated count, MCV, MCH, MCHC), immunological indices (Serum Lysozyme activity, Serum bactericidal activity, serum total protein and Serum immunoglobulin) were compared among groups. Results showed that most of haematological parameters didn’t change in anaesthetic treatments (P>0.05). Leukocyte differentiated count showed significant differences among groups (P