Micropropagation of Rosa canina Through Axillary bud

In vitro propagation has proven a suitable method for mass multiplication of uniform and diseases-free plants and acts as a new tool for modern breeding through genetic manipulation. This experiment was conducted to study the in vitro effects of growth regulators on proliferation and rooting ability of Rosa canina. Auxiliary buds were used as explant in this experiment. The stem formation was tested using completely randomized factorial design. MS medium for shoot proliferation was supplemented with various concentrations of BA (0.5, 1, 1.5, 2 mgl-1) and Kin (0, 0.5 mgl-1). Analysis of variance showed that shoot number and shoot height were highly significant at 1% level. The most shoot proliferation was observed at 1 mgl-1 BA with 0.5 mgl-1 Kin. Maximum plantlet length was obtained with 0.5mgl-1 BA and 0.5mgl-1 Kin in combination. Number of roots, ratio of root number to root length and root length showed statistically significant difference in response to different root induction treatments. Furthermore, best root regeneration was obtained at 1.2 mgl-1 IBA in R. canina. All hardened plantlets were transferred to commercial rose greenhouse.