Effect of Chitosan Nano-Gel/Emulsion Containing Bunium Persicum Essential Oil and Nisin as an Edible Biodegradable Coating on Escherichia Coli O157:H7 in Rainbow Trout Fillet

This research aimed to assess the effect of biodegradable coating chitosan nano-gel/emulsion loaded by Bunium persicum essential oil and nisin on E. coli O157:H7 in rainbow trout fillet during 12 days at refrigeration (4˚C). Trout fillet Sample was divided into 6 groups after inoculation of bacteria (E. coli O157:H7), including control (without any coating), coated with chitosan 2% and other groups including nano-emulsion chitosan 2%, Nano-emulsion of chitosan containing Bunium persicum essential oil (0.5%), Nano-gel of chitosan containing nisin (200 IU/g) and Nano-gel/emulsion of chitosan containing Bunium persicum essential oil (0.5%) and nisin (200 IU/g). The samples were stored at the cool condition, and the bacterial count was performed on days: 0, 1, 2, 4, 8, and 12. The mean number of the bacterial count was significantly different among treatment (p<0.001). The most significant inhibitory effect on the growth of E.coli O157:H7 was observed in chitosan Nano-emulsion coating containing Bunium persicum essential oil (0.5%) and nisin (200 IU/g). According to this study, it was concluded that the use of Nano-gel/emulsion of chitosan coating Bunium persicum essential oil and nisin could be effective on the decrease of E.coli O157:H7 growth in food.