Identification of genes related to the semi-compatibility characteristic between Rizobium leguminosarum strains and Lotus burttii by GWAS method

The co-existence of plant and bacteria with nodule formation and nitrogen fixation has a great effect on plant production, as well as plant protein content. In this study, 194 strains of semi-symbiotic Rizobium leguminosarum were used to identify bacterial genes related to nodule formation on L. burttii. Nodule related traits (number of white nodes, number of pink nodes, and total number of nodes) were measured at 7, 14, 21 and 28 days after inoculation. Principal Component Analysis (PCA) showed complete separation between strains with genomes A, C and D. In addition, the results of genome-wide association study (GWAS) showed that operon with secretory factor activity is effective in inducing resistance to red node trait.Doing such a job is very hard to understand an I prefer to have nothing for reaching to two hundred words until I can have green light for transferring to the next step of sending my manuscript. Sorry for that.