Detection and Genotyping of HPV Infection Using a New Method Based on Real-Time PCR

The causalGiven the known relationship between the HPV infection and some malignancies, it is critical to develop methods for quick detection and quantitation of certain HPV types while encountering a suspected lesion. Early HPV detection is greatly important in monitoring and treating the disease development and progression.  Detection of the viral DNA using PCR is the standard, noninvasive method for detecting cervical HPV infection. In the present study, we intended to develop a TaqMan genotyping assay that targets two types of high-risk HPV types (HPV 16 & 18) and two of the low-risk types (6 & 11). The study included 75 samples positive for HPV, of which 37 were positive for HPV types 16 and 18, while 38 were positive for HPV types 6 and 11. The samples had been confirmed by a reference kit before. The samples underwent real-time PCR. Each reaction consisted of the 1X CAPITAL™ qPCR Probe Master Mix, specific primer pairs for HPV, and fluorescent-tagged probes. According to our findings, all the samples genotyped using this method were compatible with the results by the reference kit, which was remarkable. In conclusion, our type-specific approach based on real-time PCR could detect the entire samples positive for four types of HPV.