The effect of topical quercetin loaded liposome on pressure ulcer healing in rats

Quercetin antioxidant properties could play an important role in various fields of health. However, its use has been limited because of several disadvantages such as very low solubility in water and high instability in the presence of air, light and heat. Encapsulation of quercetin in nanostructure systems such as liposome may lead to decrease the adverse effects and protect this molecule against degradation. The aim of this study was preparation and in-vitro and in-vivo evaluation of liposomes for topical delivery of quercetin to improve the pressure ulcers. Liposomal formulations were prepared by fusion method and characterized. The amount of drug retained in and penetrated through mouse skin after 8 hours were determined. Also microscopic and macroscopic examination of laboratory animals was performed.  Encapsulation efficacy of liposomes was in range 64.66-77.83%. Formulation F4 showed maximum drug release in 8 hours and the remaining drug in the skin layers was more than 46%. Histological investigation suggested that F4 and phenytoin 1% cream have the healing effect on the pressure ulcer during 28 day-treatment. Quercetin liposomes due to its natural structure and minimal systemic absorption and side effects can be a suitable candidate for the treatment of pressure ulcers.