Interleukin-10 Promoter and the CCR5 Polymorphisms in Iranian Azari Population with Multiple Sclerosis

Changes in the expression of cytokines as the result of the single nucleotide polymorphisms (SNPs), can affect the occurrence of multiple sclerosis (MS).

To investigate the frequency of interleukin-10 (IL-10)-1082 A/G (rs1800896) and the CCR5-delta32 genotypes on susceptibility to MS in the Iranian Azari population.

IL-10-1082 A/G SNP and the CCR5-delta32 in 152 patients suffering from MS and 242 healthy non-relatives were genotyped by allele specific-PCR and simple PCR methods, respectively.

The frequencies of AA (37.6%) and AG (55.9%) genotypes of IL-10-1082 were significantly high in the control (p = 0.021) and MS patients (p = 0.015), respectively, with no statistical difference. There was no significant difference in the CCR5 gene based on the possession of wild/wild and wild/del32 genotypes between MS patients and the control group. The del32/del32 genotype was not seen in any of the investigated groups. Tobacco (cigarettes and hookahs) consumption was higher among the MS patients (p=0.004), and this has the potential to raise the risk of MS in both the individuals and their families. It had no significant relation with the frequency of different genotypes of the IL-10-1082 and the CCR5. MS disease was more prevalent among people with low-income. (p=0.0001).

Our finding concludes on possible role of AA genotype of IL-10 -1082 as a protective factor in studied patients.