Protective Effect of Minocycline on Bax and Bcl-2 Gene Expression, Histological Damages and Oxidative Stress Induced by Ovarian Torsion in Adult Rats

Minocycline is a widely used bacteriostatic antibiotic with various functions. The aim of this study wasto investigate impact of apoptotic genes in ovary of the torsion/detorsion treated rat model by minocycline. This experimental study was performed in 32 female Wistar rats classified in four groups,including: i. sham, ii. TD: torsion/detorsion group received normal saline, iii. TDM: torsion/detorsion group treatedwith 40 mg/kg Minocycline, and iv. MC: healthy group received 40 mg/kg Minocycline. After treatment period (7days), histoplogical parameters, oxidative stress markers and hormone profile of serum as well as the expression ofBax and Bcl-2 genes were measured in the ovary of rats. Levels of superoxide dismutase (SOD), glutathione peroxidase (GPX) and estrogen were decreased in theTD group and significantly increased in the treated groups (P=0.001). Levels of malondialdehyde (MDA) and testosteronewere increased in the TD group and decreased in the treated groups (P=0.001). Expression level of Bax waselevated in the TD group, while it was attenuated in the treated groups (P=0.001). Expression level of Bcl-2 wassignificantly increased in treated groups (P=0.001). Minocycline can repair oxidative damage in ovarian tissue and regulate apoptotic-related gene expressions.