Protein-Protein Interaction Network Study Reveals New Possible Endocrine Disrupting Chemicals (EDC) ‘ Impact on Retinoblastoma

Retinoblastoma (RB) is a retinal-derived embryonic tumor. The illness severity at the time of presentation affects survival and preserves eyesight. The purpose of this research is to find Retinoblastoma causing genes and EDCs that control gene expression. To uncover genes linked to Retinoblastoma , We utilized the GEO database and a string database to rebuild the protein-protein interaction network. Then, using Cytoscape and the Clusterviz plugin, modules are explored. We utilize the Comptox database to find potential Endocrine Disrupting Chemicals (EDC) then we introduce high-degree EDCs in this network. These findings might aid researchers in better understanding melanoma.

To further understand retinoblastoma etiology and uncover possible Endocrine Disrupting Chemicals(EDCs), we looked at differential gene expression in retinoblastoma and normal samples. Then we choose differentially expressed genes (DEGs) with an adj P value of less than 0.01. The STRING database was used to create the protein-protein interaction network, which was then presented using the Cytoscape and the Clusterviz plugin was used to explore the modules. The genes in the modules were then investigated for GO and pathway enrichment. EDC-gene interactions for each module’s genes were gathered and reconstructed as a single EDC- gene network.Key words:Retinoblastoma, Protein Protein Interaction Network, systems biology, Endocrine Disrupting Chemicals.

In Retinoblastoma, 347 putative EDCs were discovered to influence gene regulation. The EDC that affects five genes is ethanolamine. For the first time, these EDCs have been suggested as new possible compounds for preventing Retinoblastoma and should be investigated further in clinical trials.

Ethanolamine is a high degree EDC in our network. However, the findings should be experimentally validated to produce better prevention.