USP7 and SET9 Expression in The Oligospermic Human Semen: A Case-Control Study

Oligospermia is defined as a less than 15 million per milliliter sperm in each ejaculation of semen. Proper and complete spermatogenesis requires the expression of a large number of genes. As a result, stopping the expression of any of them may lead to disrupt the process of spermatogenesis. In order to understand the disorders of spermatogenesis, it is necessary to study expression of effective genes in the spermatogenesis process. Therefore, in the present study, USP7 and SET9 (SETD7) gene expression was examined in the healthy and oligospermic men.

In this case-control study, semen samples of individuals with normal sperm and oligospermia were collected from men who referred to the Roya Clinic (Qom, Iran) according to World Health Organization (WHO) parameters after obtaining consent. Then the expression of USP7 and SET9 genes in two groups was analyzed using quantitative polymerase chain reaction (qPCR).

There was no difference forage between the healthy and oligospermic individuals (P=0.889). The data showed that, USP7 gene expression in the patients was 3.99 times higher than the control group (P<0.001). The expression of SET9 gene in the patient was 1.28 times less than the control group, which was not significant (P=0.231). The results indicated that USP7 gene expression was increased in the 84% of oligospermic individuals.

The USP7 gene can be considered as one of the molecular markers in the development of oligospermia.