Vitamin D Binding Protein Gene Polymorphisms and its Association with Type 2 Diabetes Mellitus in an Iranian Population

Polymorphisms of Vitamin D-binding protein (DBP) may represent a risk factor for susceptibility to Type 2 Diabetes Mellitus (T2DM). Two polymorphisms are common at codons 420 (ACG to AAG) and 416 (GAT to GAG) in the DBP gene. The present study aimed to assess variants of DBP at codons 420 and 416 utilizing polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP), which were digested by Sty I (codon 420) and Hae III (codon 416) restriction enzyme. For this purpose, 240 patients were recruited along with 159 controls. The genotype frequency of Glu/Glu at codon 416 in control and patient groups was 25.16% and 32.92% respectively. Moreover, the genotype frequency of Lys/Lys was 6.67% in patients and 1.89% in controls at codon 420. A significant difference was found between control and patient groups in genotype frequencies at codon 420 (p<0.05). It was found that there might be an association between the vitamin D-binding protein gene and T2DM.