Biodegradability of Diazinon Poison in Grand Soils by Bacterias Isolated from them
Diazinon is an organophosphorus insecticide that is widely used in paddy fields and gardens. The entry of resistant pollutants into drinking water sources, soil, and agricultural products can be harmful to human health and the environment. This study aimed to identify and isolate diazinon-degrading bacteria and measure the amount of poison decomposed by superior bacteria in liquid and soil environments.
Sampling was conducted from Tonekabon gardens. The strains were identified and sequenced using polymerase chain reaction based on 16S rRNA. The rate of diazinon degradation by the isolated bacteria was measured by gas chromatography
Two strains of diazinon-degrading bacteria, Serratia and Enterobacter cloacae, were identified. Bacteria with diazinon concentrations of 10 and 20 ppm were cultured in broth and soil for 10 days, and the amount of poison decomposition was measured. The results indicated that Serratia bacteria in the broth medium, with initial concentrations of 10 and 20 ppm, consumed 64.3% and 78.4% of diazinon, respectively, while in the soil with the same concentration, it decomposed 90.2% and 98.25% of diazinon. Enterobacter cloacae bacteria consumed and decomposed diazinon in the broth and soil environments, with percentages of 23.1, 17.95, 31.19, and 88.05%, respectively. According to the results, Serratia has a higher decomposition ability compared to Enterobacter.
The results of this research showed that some microorganisms in the soil have the ability to decompose diazinon. Utilizing these microorganisms and other biological methods to clean contaminated soils can be a suitable approach.
Bacteria , Soil , Diazinon , Biodegradability , Serratia
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