Study on some Stevia ( Stevia rebaudiana Bertoni ) metabolites in the invitro and invivo conditions using GC- MS

Stevia (Stevia rebaudiana Bertoni) is a perennial plant that well known due to natural sweeteners Diterpenesthat contributes 4-20 percent of dry matter of the plant's leaves. Since stevia multiplication is performed byseed, transplanting, cuttings and tissue culture, this study specifically compare the chemical compounds of theplant in different growth conditions, including natural and tissue culture conditions using plant extracts andessential oil. The results showed that among 55 identified chemical compounds in the extract of tissue culture,16 compounds were common and 39 others were exclusively in tissue culture condition. Also, among total of70 chemical compounds in the extract of natural condition, 54 compounds were exclusively observed in extractof stevia grown in natural condition. The results of GC-MS of essential oil showed that there were eight similarcompounds among natural and tissue culture samples. Therefore, among 41 identified chemical compounds inthe stevia essence obtained from tissue culture, there were only 33 of composition exclusively observed intissue culture conditions. Also, among 164 compounds identified in a plant essential oil grown in a naturalconditions, 156 of them were exclusively observed in natural conditions.