The Impact of a Single Exhaustive Training Session with Protein Supplementation on Muscle Damage Markers in Young Cyclists

This study aimed to investigate thei of a single exhaustive training session with protein supplementation on muscle damage markers in young cyclists. Twenty professional cyclists with experience at local and national championship levels were recruited using a convenience sampling method. They were divided into an experimental group (n=10; mean age 21.4±4.7 years; body mass index (BMI) = 20.56±2.12 kg/m2) and a placebo group (n=10; mean age 19.1±4.1 years; BMI = 21.48±3.96 kg/m2). Blood samples were collected in a fasted state to measure phospho creatine kinase (CPK) and lactate dehydrogenase (LDH) levels. The experimental group consumed a solution containing 400 mg of protein powder (100 mg per kilogram of body weight) with 400 cc of water, while the placebo group consumed a solution containing maltodextrin powder (100 mg per kilogram of body weight). A second blood sample was taken one hour after supplementation. Participants did not engage in any physical activity during the supplementation period. After a 10-minute warm-up, the Wingate test was performed, and blood samples were collected 24 and 48 hours after the test. The findings indicated that one exhaustive training session with protein supplementation did not have a significant effect on CPK (p=0.126) and LDH (p=0.526) concentrations in professional cyclists. Post-CPK and LDH concentrations were highest at 24 and 48 hours after strenuous activity. Based on this study, a single exhaustive training session with protein supplementation did not significantly impact muscle injury markers in young cyclists. Therefore, alternative nutritional recommendations and strategies should be considered.