The use of Artemia Nauplii enriched with espresso and docosahexaenoic acid in the post-larval stage of the Vannamei shrimp (Litopenaeus vannamei)

This research aimed to enrich Artemia nauplii with Easy DHA and Espresso to improve the growth and survival of vannamei shrimp post larvae and transfer them to brackish water (Caspian Sea). 2100 vannamei shrimp post-larval with an approximate average weight of 49.54 ± 2.60 mg and an average length of 1.09 ± 0.07 cm (PL8) were selected. For each replication, 50 vannamei shrimp post-larval were randomly placed in forty-two 20-liter glass tanks (containing 15 liters of water) and were distributed indoors. Post-larvae were fed, including freshly hatched Artemia, Artemia enriched with Easy DHA and espresso, and commercial diet for ten days (in salt water and desalination water). The highest growth rate (weight and length) in the saltwater group was observed in treatment seven (P < 0.05). Also, the highest survival rate in this group was observed in group three, with 71% (P < 0.05). Among the treatments of the brackish water group, the highest growth rate (weight and length) was observed in group fourteen (P < 0.05). The highest survival rate was observed in treatments of nine, ten, and thirteen (P < 0.05). Post larvae of the sea and brackish water showed the highest amount of fatty acid C20-5n3 in treatment three and fatty acid C22-6n3 in treatment two (P < 0.05). Based on the present study's findings, enrichment of Artemia with Easy DHA and Espresso and feeding post larvae with it for nine days can increase survival and resistance to stress (transition to lower salinities) in vannamei shrimp post larvae.