Investigation of the expression pattern of some nitrogen transporter genes in root in two different cultivars of wheat

In crop production, one particularly limiting factor is the availability of nitrogen (N) and N derivatives, as they are of much significance in plant metabolism and growth. Various nitrate transporter genes mediate the uptake, transport, and remobilization of N in the plants. However, their role is not quite understood with respect to grain filling stages and plant’s overall yield. Herein, the expression of a number of genes and enzymes with roles in N uptake and metabolism was measured over the course of anthesis and under N-treatment and N-deficit condition in two wheat genotypes, namely Morvarid and Gonbad. The samples were taken 10, 15, and 20 days after anthesis, which is a critical stage in grain filling, and the relation between gene expression, enzyme activity, and days after anthesis was demonstrated. It was observed that N-related gene expression significantly increases under N-deficit condition and over the course of anthesis, suggesting the significance of the N-related genes and enzyme in maintaining the metabolism and growth of the plants under N-deficit conditions.