Influence of mechanical milling time on physicochemical properties and stability of cefotaxime sodium

The aim of this study was to examine the effect of mechanical milling time on physicochemical properties and stability of Cefotaxime sodium (CS). CS was micronized by ball milling in five period of time: 30, 60, 120, 240, and 360 min. The powder properties of the samples were examined by HPLC assay, laser diffraction, helium densitometery, IR spectrophotometery, X-ray diffraction (XRD), scaning electron microscopy (SEM), differential scanning calorimetery (DSC), thermogravimetric analysis (TGA) and Karl-Fisher titrimetery. The results showed that ball milling was not an appropriate method for particle size reduction to make solid dosage form such as dry powder inhaler formulation (DPI) of CS and by increase in milling time, degradation of CS increased.