Study effect of garlic choloroformic extract (Allicin) on physiology and morphology of brucella

Brucella under effects of agents such as penicillin, glycin and some hormones could convert to L-form. L-forms are possible during disease or latent brucellosis and interfering in renew relapsing disease. Garlic is a medicine plant that allicin the most important antimicrobial, is its compound. In this study after garlic choloroformic extract prepared and quantity of allicin calculated; MIC and MBC for two strains (B.melitensis Rev1 and B.abortus S19) was calculated. Effect of extract on Brucella morphology in subMIC concentrations; effect of time and temperature on extract activity and antimicrobial effect of extract with sucrose were studied. Results indicated that antimicrobial effect of allicin is temperature independent and showing its effect on bacteria in two hours. Also L-forms were not isolated in subMIC concentrations.