Use of Image analysis system to Karyotype of Trifolium

A karyological study of six species: Trifolium campestre, T. dasyurum,, T. grandiflorum,, T. lappaceum,, T. resupinatum, and T. repens is presented.We used Video Analysis system for each species with Micromeasure software. Some features of chromosome such as SA, LA, TL, AR and CI were determined. For assignment of evolutionary situation of all species, except T. repens that was tetraploid others were diploid and we found the two basic number in the genus: x=7,8. we determine karyotype symmetric parameters such as A1, A2, %TF and DRL. The data for all traits on each species were analyzed as RCD experiment and the results showed all species had significant difference for all traits at %1. This results were expressed variation chromosomes in all species. Using Stebbins method karyotipical characters were identified and it was shown that all of species are in 1A class.. Finally the 6 species were grouped in to 3 cluster.