فهرست مطالب

Personalized Medicine Journal
Volume:4 Issue: 16, Winter 2020

  • تاریخ انتشار: 1398/11/15
  • تعداد عناوین: 7
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  • Blnd Ibrahim Mohammed *, Saeed Megdadi, Vahid Reza Esfahani Pages 1-2
    HCV is a blood pathogen that affects approximately 180 million people worldwide. It has the ability to escape its host’s defense mechanism and is considered a resistant species. Toll-like receptors (TLRs) are a family of evolutionary receptors that have been identified by the diagnosis of pathogens as the main regulators of innate and acquired immune responses. Studies have shown that genetic changes in the TLR3 gene are associated with high susceptibility or resistance to immune and infectious diseases. In this study, the effects of the TLR3 gene single nucleotide polymorphism rs78726532 on susceptibility to HCV infections were examined. The association between this polymorphism and the risk of hepatitis C in 50 patients and 50 healthy subjects was investigated. The results revealed a significant relationship between polymorphism rs78726532 and hepatitis C infection (p = 0.002). Thus, it could have a therapeutic and predictive potential.
    Keywords: Hepatitis C, Toll- like receptors, single nucleotide polymorphism
  • Ramadhan Ibrahim *, MohammadAli Saremi, MohammadAli Keshavarz Shahbaz, Maryam Forouhi Pages 3-5

    Because of their small size, unique physics, and chemical properties, metal nanoparticles can easily cross obstacles and reach their target cells, which makes them an ideal choice for therapeutic purposes in various cancers. In this study, the effects of iron oxide nanoparticles on MDA-MB-231 breast cancer cell line were examined, and biomarkers related to oxidative stress were evaluated. Fe2O3 nanoparticles were suspended in a cell culture medium and diluted to appropriate concentrations (0, 10, 30, 60, and 120 μg/ml) for 24 and 48 h. GSH, superoxide dismutase, catalase, and ROS generation were evaluated. The results showed that iron oxide nanoparticles induced intracellular ROS generation in a dose- and time-dependent manner. The results further showed that iron oxide nanoparticles increased ROS and activated oxidative stress in cells.

    Keywords: Iron oxide nanoparticle, Oxidative stress, Reactive Oxygen Species, Breast cancer
  • Fawziah M. Mohammed *, Amir Mohammadi, Najme Shojaei Pages 6-8
    In many cancers, an increase in gene expression of DNMT3b has been reported. Thisenzyme inhibits the expression of many tumor suppressor genes through the methylation ofthe promoter sequence and plays a key role in the progression of cancer. In this study, therelationship between polymorphism rs406193 of this gene and the risk of prostate cancerin 60 samples was investigated. The results showed a significant association betweengenotype TT and the risk of prostate cancer (p=0.048). It is recommended that this studybe repeated in larger populations and that the relationship between this polymorphism andgene expression be investigated.
    Keywords: prostate cancer, Epigenetic, single nucleotide polymorphism, DNMT3b
  • Massoud Houshmand *, Mehrshad Seresht Ahmadi, Mahsa Alikarami Pages 9-11

    Obesity and type 2 diabetes are two complex disorders with strong genetic backgrounds, and both disorders cause other diseases and increase mortality. FTO is a protein-coding gene located on the long arm of chromosome 16 and linked to type 2 diabetes and obesity. The current study investigated the relationship between the RS16953002 polymorphism gene and type 2 diabetes and obesity in a population from Tehran. The study population included 150 people with type 2 diabetes and 150 healthy individuals. Genotyping was performed by RFLP-PCR. The results indicated the presence of the GG allele in 81% of diabetic patients but in only64% of non-diabetic participants, which shows a statistically significant difference in this regard. Thus, the current study has shown the role of FTO gene polymorphisms in the pathogenesis of people with type 2 diabetes and obesity.

    Keywords: single nucleotide polymorphism diabetes FTO RFLP, PCR Genotyping
  • Abdulkarim Karim *, Hadi Yari, Ehsan Razeghian, AliReza Miri Lavasani Pages 12-14

    Cervical cancer is the second most common cancer and an important cause of death in women worldwide. Objective biomarkers are needed to improve specificity for cervical cancer screening. The p16 gene is implicated in the cell cycle control, playing an important role as a tumor suppressor gene. In this study, the methylation of the P16 gene promoter was evaluated in people with cervical cancer and people with the papilloma virus. The study population included nine women with cervical cancer whose malignancy had been confirmed by a pathologist and ten patients with high-risk types of HPV virus. Methylation status was evaluated by MS-PCR. Cervical cancer patients showed a significantly higher methylation frequency for the p16 gene as compared to the control and the HPV group (p=0.001).

    Keywords: Methylation, Epigenetic, cervical cancer, papilloma virus
  • Kazhaleh Mohammadi *, Mehrdad Tavakoli, Nafise Poorhasan, Maryam Forouhi Pages 15-16
    According to the World Health Organization, one in eight to ten women will develop breast cancer. Therefore, the development of new blood / serum markers is important as an alternative to traditional diagnosis methods. Early detection of breast cancer plays an important role in choosing the right treatment approach and treating the patient. In this study, the expression of miRNA21 gene in the plasma sample of people with breast cancer compared to healthy people was evaluated. The results of qPCR showed that the expression levels of miRNA-21 in the plasma samples of breast cancer patients were significantly increased compared to those of the healthy controls. These results suggest that Mir 21 could be a very good blood biomarker for the early detection of breast cancer.
    Keywords: Breast cancer, blood biomarker, MicroRNA-21, Gene expression
  • Sahar Hassannejad *, Reza Ghamari, MohammadAli Saremi, Sadegh Shojaei Baghini Pages 17-19

    Spermatogenesis is a very complex process that can be affected by a number of factors which may lead to reduced fertility or infertility. Busulfan kills spermatogonia stem cells and disrupts the connections between Sertoli and spermatogonia cells at base layers. Moreover, vitamin D is very important in the reproductive system of men. In this study, the effect of vitamin D on biosulfur-induced azoospermia in mice was investigated. The results showed an increase in the quality of sperm parameters in the group treated with vitamin D.

    Keywords: Vitamin D, Cancer, Busulfan, infertility, Sperm parameters