Journal of Basic and Clinical Veterinary Medicine
Volume:3 Issue: 1, Sep 2022

This study conducted to evaluation of antibiotic resistant and molecular identification of Escherichia coli isolates from poultry colibacillosis in Tabriz city during 2020. Forty-six  Escherichia coli samples from colibacillosis cases of 10 aviculture in Tabriz city, which had been previously isolated and detected by microbiological and biochemical methods, were identified molecularly with 16s rRNA primers by PCR method. To determine the pattern of antibiotic resistance of isolates, common antibiotics available in the market were used by Kirby-Bauer method. Of 46 Escherichia coli samples tested, 40 isolates (86.9%) were identified as Escherichia coli. All strains of Escherichia coli (100%) were resistant to ampicillin. The most strains were resistant to ceftriaxone (75%), neomycin (70%) and nitrofurantoin (70%). Twelve Escherichia coli strains (30%) were resistant to all of tested antibiotics. It can be concluded that the antibiotic resistance of Escherichia coli isolates to common antimicrobial drugs used in the poultry industry is high. So, implementation of national monitoring programs is strongly needed for antimicrobial resistance and rational use of antibiotics.

Nowadays, ornamental fish are considered to be of value in most countries due to their beauty and variety. The living, beautiful and lively environment of aquariums has made people to be interested in having an aquarium, however small in size, at their home or work. The main aim of this study is to identify the parasitic elements in ornamental fish (92 Goldfish , 32 Oscar fish, 18 Flower fish, 24 Arvana fish, and 41 Discus fish) that are culled from fish breeding centers in Urmia. Classification of the fish was carried out based on their morphological characteristics. The results of the study indicate the presence of parasitic infection of these fish in four protozoan species including Ichthyophthirius multifiliis, Trichodina spp, chilodonella spp and Hexamita spp. Also, Diplostomum spataceum, Dactylogyrus spp and Gyrodactylus spp were observed from monogenea and digenea trematodes and lernaea spp from crustose parasites.

The aim of this study was to evaluate the changes in the biochemical parameters in infected dogs with babesiosis in 3 levels. The Babesia genus, as pathogen blood parasites, cause financial problems in livestock industry. Many published papers have suggested babesiosis-induced biochemical parameters, but no study has determined these parameters at four levels of parasitemia (less than 1%, 1%, 2% and 3%). Dogs with babesiosis were identified based on clinical signs and the observation in red blood cells. After blood sampling from 38 infected and 38 healthy dogs, biochemical parameters glucose paraoxonase, superoxide dismutase, catalase, glutathione peroxidase and adenosinedeaminas were measured in plasma. The results indicated significant increase and decrease in biochemical parameters. In conclusion, the results suggested that dog babesiosis causes cellular damage, especially in kidney and anemia in infected dogs. Furthermore, babesiosis caused kidney damage.  This infection affected the activity of liver enzymes. The obtained results revealed a highly significant increase in serum Aspartate aminotransferase and Alanine aminotransferase.

Primary zinc deficiency is not common in ruminants, but its occurrence has been documented. This study was conducted on a flock of 45 Kermani sheep during February 2022 to June 2022. The signs of alopecia, pityriasis, infertility, growth retardation, weakness, anorexia, itching, pneumonia, conjunctivitis, and increased risk of infection, which was suspected of zinc deficiency, were observed in 4 sheep. The heart rate, respiration rate, body temperature, and blood indices including PCV, total and differential white blood cell count, and red blood cell count were measured. Hematology results indicated a significant increase in white blood cells in affected sheep. The achieved biopsied cutaneous tissues of the sheep with skin lesions, were stained by Hematoxylin and Eosin method. Pathologically, the parakeratosis was observed in cornified squamous epithelium and therefore zinc deficiency was confirmed. The treatment strategy included oral zinc sulfate tablets with a dose of 250 mg per day for 4 weeks and the use of mineral bricks to continue the treatment process. After 10 weeks, signs of recovery appeared.

Sebaceous gland neoplasms are one of the cutaneous neoplasms in the canine. A 5-year-old ovary hysterectomy female, mixed breed terrier was referred to veterinary clinic of the Islamic Azad University, Shahrekord Branch due to a mass in the head area. The morphological characteristics of the lesion revealed an encapsulated, round, solitary yellowish-white mass, relatively soft consistency with an approximate diameter of 9 mm in the right midline the forehead and calvarium area with minor hair loss. Next, a complete excisional biopsy was done for pathology diagnosis. After dehydration, clearing and impregnated with paraffin, a tissue sample was stained with Hematoxylin and Eosin method. The microscopic characteristics of the tumor mass showed two different cell types, including mature sebaceous gland cells with vacuolated cytoplasm and small hyperchromic nucleus, and several rows of basaloid cells around sebaceous glands without mitotic division that were not adjacent to hair follicles. Inside several follicles, sections of the middle parts of demodex's body were observed. Our report is a unique example of sebaceous adenoma with infection of hair follicles with demodex mites in an ovariohysterectomy in a female dog.

One hundred and fifty samples suspected of coli bacillosis were isolated from broilers; laying hens and mother hens were sampled from 21 farms. The test was positive in 98 cases. And their sensitivity to ten types of antibiotics was investigated using antibiogram method, the disc diffusion method and the growth inhibition halo diameter in (mm). Ninety positive cases (60%) were meat breeding chickens and 8 positive cases (5.33%) were mother and laying hens. The antibiotic discs were related to tetracycline, ampicillin, difloxacin, enrofloxacin, doxycycline, sulfamethoxazole + trimethoprim, ciprofloxacin, calcitin, florfenicol, Fozbak. The biggest halo was related to florfenicol and Fosbak (21-22 mm). Also, the smallest halo was related to tetracycline and ampicillin (8-9 mm). Average halo diameter among ten antibiotics was measured from less than 8 mm to more than 22 mm. . All isolated Escherichia coli were at least 26% resistant to 7 types of antibiotics. Old generation antibiotics such as tetracycline and ampicillin were less effective than new generation antibiotics. In conclusion, the frequency of antibacterial resistance was high due to their excessive consumption and it would be a serious risk for the country's poultry industry and public health.