فهرست مطالب فرزانه ضرغام پور

Today, cancer stem cells have attracted a lot of attention as tumour-initiating cells. Hyperthermia was also found to be beneficial in cancer treatment in many studies. In the present study, we assessed the effects of hyperthermia on colorectal cancer cells (HT-29) and their derived cancer stem cells (CSCs).

 CSCs were isolated from the HT-29 cell lines and then, the expression of surface marker and stemness-related genes were evaluated. Next, the toxicity effect of hyperthermia on HT-29 and CSCs was assessed. In addition, the effect of hyperthermia on the expression of stemness, proliferation, Wnt signaling pathway, and apoptosis genes was evaluated. Finally, the data were statistically analyzed.

 Isolated-CSCs were positive for CD133 surface marker and the expression of stemness genes in the isolated-CSCs was significantly higher than HT-29 cells (P-value< 0.05). Treatment with hyperthermia reduced the survival rate of HT-29 and CSCs. In both groups, the gene expression of OCT3/4, NANOG, PCNA, WNT1, and CTNNB1, was decreased (P>0.05). In addition, the expression of P53, KLF4, and BAX genes was significantly increased in CSCs (P <0.05).

 The results showed that hyperthermia did not have a significant effect on HT-29 cells. However, it had a significant effect on the cell viability, growth curve, expression of genes involved in apoptosis and KLF4 in treated-CSCs. Therefore, hyperthermia can have a beneficial effect on inhibiting the growth and proliferation of CSCs.

Osteoporosis is the most common disorder in metabolism of bone minerals. Restriction site Taq1 in Calcitonine gene and AluI polymorphism in Calcitonin receptor gene as genetic factors can be associated with bone mineral density. The aim of this study was to determine the association of restriction site Taq1 and AluI polymorphism with bone mineral density. In this descriptive analytical study, 200 women over 45 years who referred to Bone Mineral Density Assay Centers in Shahrekord in years 1391-92 were included. Bone mineral density at femur neck and lumbar spine was measured by DEXA method. Based on T score, an indicator of bone mineral density these women divided to patient or osteoporotic group (130 persons) and healthy or control group (70 persons). Then different genotypes of Taq1 (TT/Tt/tt) and AluI (TT/TC/CC) were determined by PCR-RFLP method and data was analyzed by statistical tests, including Chi square and ANOVA. P values less than 0.05 was considered statistically significant. In control group the percent of genotype distribution for TT, TC and CC genotypes was 31.4%, 35.7% and 22.9%, respectively. In patient group the genotype distribution percentage for above genotypes was 31.54%, 63.08% and 5.38%, respectively. The P value of this polymorphism with bone mineral density was more than 0.05. Both in control and patient groups for Taq1 restriction site the tt genotype was seen. There was no association between each of AluI polymorphism and Taq1 restriction site and bone mineral density and it is possible that other genetic factors would be effective.